非致病性尖孢镰刀菌固体发酵条件筛选

摘要

Optimal culture conditions on solid-medium for the non-pathogenic Fusarium oxysporum FJAT-9290 to produce vaccine against fusarium wilt were investigated.The initial moisture content and inoculum amount in the medium,as well as the temperature and duration for the fermentation were varied to determine their effects on the resulting yield of conidia.It appeared that,a medium with 50％ moisture in a tissue culture bottle inoculated with 7％ of FJAT-9290 to ferment at 28℃ for 7 ds followed by cultivation in a polypropylene bag could produce a maximized conidium count of 6.35 × 108 · g-1 on the 20th day.The condia count quadrupled that of the initial inoculum.%为优化枯萎病植物疫苗工程菌株——非致病性尖孢镰刀菌FJAT-9290固体发酵条件,以产孢量为指标,分析了培养基初始含水量、初始接种量、培养温度和培养时间对菌株产孢量的影响,筛选出最优的发酵条件.结果表明,利用组培瓶培养7d,分别筛选出菌株生长的最佳初始含水量为50％、初始接种量为7％和培养温度为28℃,并在此基础上,利用聚丙烯塑料袋培养,发酵至20 d时菌株的产孢量最高,孢子数量达6.35×108个·g-1,较初始接菌量增加了4个数量级.