196份福建葡萄品种资源亲缘关系的ISSR分析

摘要

利用ISSR-PCR分子标记技术对福建省各葡萄种植区收集到的196份葡萄资源进行亲缘关系鉴定.试验结果表明,12条引物可以清晰扩增出164条谱带,多态性位点的平均百分率为91.44％;聚类分析表明,196份葡萄资源可以分为3大类,即东亚种群、欧美杂种和欧亚种;群体遗传分析表明,葡萄资源种内存在丰富的遗传多样性.%Genetic relationships among 196 grape cultivars collected from various regions in Fujian were determined using ISSR-PCR.The 12 selected primers amplified 164 bands from the plants with up to 91.44％ polymorphic loci.The clustering analysis revealed that the germplasms could be divided into three categories as the eastern Asia population,the inter-specific hybrids (Vitis vinifera V.labrusca) and V.vinifera.A population genetic polymorphism analysis on the cultivars showed abundant diversities among them.