THE METHOD FOR DETERMINATION OF PHYLOGENETIC RELATIONS AMONG STAPHYLOCOCCUS SPECIES STRAINS

摘要

The new method for species determination and intra-species diversity analysis of Staphylococcus strains was revealed. An exemplary way to achieve this is the use of the polymerase chain reaction (PCR) to obtain the product on the conserved locus template, which is subsequently digested with restriction enzymes (method known as restriction fragments length polymorphism, RFLP). This allows to obtain restriction patterns allowing for species determination. Intra-species diversity analysis for staphylococci is also possible when the RFLP is carried out for properly selected enzymes.