The grapefruit seed extract (GSE), a natural plant extracts from the edible plants used as an extremely potent broadspectrum bactericide, and fungicide should be very effective for the fruit preservation. In this study, Grape (Vitis vinifera L.) and persimmon (Diospyris kaki L.) easily undergone deterioration, were selected as model fruits. Eight fungi familiar to the rotting cause of grape and persimmon were used to testify the antifungal activity of GSE. The results of antifungal assay by the agar diffusion method indicated that GSE can efficiently inhibit the growth of tested fungi. The values of minimal inhibitory concentration (MIC) also range from 39.06×10-6 to 625×10-6 as demonstrated by the similar results with the data taken from agar diffusion assay. In the storage application assay of both grape and persimmon, the obvious differences in sensory quality and microorganism indexes between the GSE treated fruits and control fruits support that GSE has both the strong antifungal activity and antioxidative activity. Polyphenolic compounds, rich in GSE used as the main effective antioxidative component,are capable to prevent fungal infection in storage to a large extent and to postpone ripening and aging of fruits effectively by scavenging the free radical and impacting a series of enzyme relating to postharvest physiological metabolism of fruits, particularly in the key parts liking stem and calyx. The obtained results also indicated that GSE can decrease browning and rot ratio,prolong the preservation period, ensure the quality of grape and persimmon and can be used as an effective and safe preservative.%葡萄柚种子提取物是潜在的广谱性细菌、真菌杀菌剂,应该在果蔬保鲜中具有很好的效果.本研究选取比较容易受真菌感染的葡萄和柿子作为模式保鲜材料并且测试了葡萄柚种子提取物对8种与这两种水果腐败相关的真菌的抑制效果.结果表明,这8种真菌的最小抑制浓度分别在39.06×10-6至625×10-6之间不等.果实保鲜结果也证实了葡萄柚种子提取物对真菌具有很好的杀抑作用,延缓了果实的成熟,并且使保鲜的果实保留了较好的风味.这些结果可能与葡萄柚种子提取物富含多酚类物质有关,因为多酚类物质具有很强的抗氧化活性.
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