草鱼鱼鳞胶原蛋白体外自组装行为的研究

摘要

In this study,acid-soluble collagen（ASC） and pepsin-soluble collagen（PSC） were extracted from grass carp scale and molecularly characterized.Meanwhile,a preliminary investigation into their self-assembly behavior in vitro was carried out along with a comparison with porcine skin collagen（PC）.ASC,PSC and porcine skin collagen were all type Ⅰcollagens and showed a high similarity in molecular weight and secondary structure but a difference in amino acid composition.The results of turbidity tests indicated that all the collagens exhibited behavior in vitro under the appropriate temperature and pH.The process of self-assembly consisted of lag,rapid rise and plateau phases and their occurrence times were affected by medium pH.Under the same conditions,the self-assembly degree of porcine skin collagen was highest,followed by PSC and ASC.Environmental temperature,collagen concentration and thermal denaturation temperature had different effects on the self-assembly behavior in vitro of PSC.%以草鱼鱼鳞为原料,分别提取鱼鳞中的酸溶性胶原蛋白（ASC）和胃蛋白酶溶性胶原蛋白（PSC）,在对胶原蛋白分子结构表征的基础上,开展鱼鳞胶原蛋白体外自组装行为的研究,并与哺乳动物来源的猪皮胶原（PC）相比较。结果表明：制备所得的3种胶原蛋白均为典型的Ⅰ型胶原,3种胶原蛋白的分子质量和二级结构较为相似,但氨基酸组成存在一定差异。采用浊度实验的方法观察胶原蛋白的体外自组装行为,结果表明：在合适的温度和pH值条件下,3种胶原蛋白均可产生体外自组装行为,其自组装进程可划分为迟滞段、快速上升段和平稳段3个阶段,其出现时间受溶液pH值的影响;在相同条件下,PC的体外自组装程度最高,其次为鱼鳞PSC和ASC;环境温度、胶原蛋白质量浓度以及蛋白的热变性处理均会不同程度的影响胶原蛋白的体外自组装行为。