目的：优化葛渣中总黄酮提取工艺条件，测定葛渣中葛根素含量。方法：以葛渣为原料、总黄酮得率为评价指标，采用L9（3^4）正交试验设计，考察乙醇体积分数、提取时间、固料比、提取温度对葛渣总黄酮提取效果的影响，同时采用高效液相色谱法（HPLC法）测定葛根素的含量。结果：葛渣中总黄酮的最佳提取工艺条件为以体积分数70％乙醇溶液为溶剂、提取150min、料液比1：40（g／mL）、提取温度80℃，此条件下的总黄酮得率最高，为32．41mg／g。在该提取条件下，葛根、葛渣和葛粉中总黄酮的含量分别为46．11、32．4%In this paper, orthogonal array design L9（34） was employed to optimize the extraction conditions of total flavonoids from Radix Puerariae residue left after pueraria root powder extraction. The effects of extraction temperature, ethanol concentration, extraction time and material-to-liquid ratio on extraction rate of total flavonoids were probed. Meanwhile, a HPLC method to determine puerarin content in Radix Puerariae was presented based on optimized extraction process. The optimal extraction conditions were determined as follows： ethanol concentration 70%, reflux time 150 min, reflux temperature 80 ℃, and material-to-liquid ratio 1：40, under which, the contents of total flavonoids in Radix Puerariae, Radix Puerariae residue and pueraria root powder were 46.11, 32.41 mg/g and 11.23 mg/g, and of puerarin 40.63, 21.24 mg/g and 4.71 mg/g, respectively. From these results, we drew the conclusion that Radix Puerariae residue is rich in total flavonoids and puerarin and that the optimized extraction process can be used for the extraction of total flavonoids and puerarin from Radix Puerariae residue.
展开▼
