目的:建立海蒿子多糖超声波-微波协同提取工艺,提高海蒿子多糖的提取率。方法:利用硫酸-苯酚法考察正交试验在不同粒度、提取时间、料液比、超声波功率、微波功率条件下多糖提取率,选出最佳超声波-微波协同提取工艺。结果:最佳提取工艺为粒度40目、提取时间20min、料液比1:25(g/mL)、超声波功率75%、微波功率200W,其中粒度对多糖提取率的影响最大。结论:超声波-微波协同提取能缩短提取时间并提高海蒿子多糖提取率。%A high-yield method for the extraction of polysaccharides from Sargassum pallidum was proposed using an integrated ultrasonic and microwave reaction system. The extraction yield of polysaccharide was measured by phenol-sulferic acid method. The optimal conditions of raw material particle size, extraction time, solid-to-solvent ratio, ultrasonic power and microwave power for extracting polysaccharides from Sargassum pallidum were 40 mesh, 20 min, 1:25 (g/mL), 75%and 200 W, respectively, as determined by orthogonal array design. Among five operating parameters, raw material particle size was the most important factor for polysaccharide extraction. The combined use of ultrasonic and microwave provided a time-saving and high-yield method for polysaccharide extraction from Sargassum pallidum.
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