文昌鱼多糖对荷S180小鼠淋巴细胞增殖和抗氧化水平的影响

摘要

目的：研究文昌鱼多糖（PBB）的组成及其对荷S180小鼠淋巴细胞增殖和抗氧化活性的影响。方法：以酶水解法对文昌鱼多糖进行提取，并应用沉淀法对粗制品进行分离纯化；以苯酚．硫酸法测定总糖含量。样品经盐酸一甲醇醇解成单糖，采用标准对照法及内标法进行气相色谱定性定量测定。建立S180荷瘤鼠模型，随机分为PBB低、中、高剂量组（25、50、100mg／（kg·d））、正常对照组、肿瘤对照组和环磷酰胺对照组，测定PBB干预对荷S180小鼠抑瘤率的影响；MTT法检测PBB对荷S180小鼠脾淋巴细胞增殖的影响：酶学实验检测PBB对荷S180小鼠血浆超氧化物歧化酶（SOD）、还原性谷胱甘肽（GSH．Vx）活性及丙二醛（MDA）含量的影响。结果：PBB总糖含量为89．3％；PBB中单糖含量分别为鼠李糖1．04％、木糖1．01％、岩藻糖1．02％、甘露糖0．35％、葡萄糖0．56％、半乳糖2．64％。经25、50、100mg／（kg·d）剂量作用2周后，小鼠S180肉瘤生长均受到抑制，抑瘤率分别达到39．1％、47．5％、53．4％；荷S180小鼠淋巴细胞增殖率较肿瘤对照组明显提高，经统计学处理，有显著性差异（P〈0．05）；荷S180小鼠血浆MDA含量均显著降低，荷S180小鼠血浆SOD和GSH—Px活性明显升高，与肿瘤对照组比较，具有显著性差异（P〈0．05）。结论：文昌鱼中含有丰富的多糖物质。文昌鱼多糖可明显抑制S180肉瘤的生长，其作用机制可能与PBB提高机体淋巴细胞增殖，改善机体抗氧化能力有关。%Objective： To explore the monosaccharide composition of polysaccharides extracted fromBranchiostoma belcheri （PBBs）, and the effect of PBBs on lymphocyte proliferation and antioxidant activity in S180-bearing mice. Methods： Polysac- charides were isolated from Branchistoma belcheri by enzymatic hydrolysis and alcohol precipitation. The monosaccharides obtained by alcoholysis with HCI-CH3OH from PBBs were determined by gas chromatography with internal standard method. S 180-bearing mouse models were established and the model mice were randomly divided into six groups including model group, normal group, PBBs groups （25, 50, 100 mg/（kg · d）） and cyclophosphamide group. The tumor inhibition rate of S 180-bearing mice was determined. The effect of PBBs on spleen lymphocyte proliferation was evaluated by MTT assay. The contents of SOD, MDA and GSH-Px in mouse plasma were determined using test kits. Results： The purity of PBBs was determined to be 89.3%. The neutral monosaccharides included rhamnose, xylose, fucose, mannose, glucose and galactose. PBBs administration at daily dosages of 25, 50, 100 mg/kg for 2 consecutive weeks could inhibit the growth of S 180 sarcoma with inhibitory rates of 39.1%, 47.5% and 53.4%, respectively. Meanwhile, PBBs revealed an obvious reduction in MDA content and a significant increase in plasma SOD and GSH-Px activities. Conclusion： Branchiostoma belched is rich in PBBs that function to inhibit the growth of S 180 sarcoma through enhancing spleen lymphocyte proliferation and antioxidant activity in S 180-bearing mice.