Comparison of the Effects by Polysaccharides from Wood-cultured and Bag-cultured Ganoderma lucidum on Murine Spleen Lymphocyte Proliferation in vitro

摘要

This research compared the influences of wood- cultured Ganoderma lucidum polysaccharides (GL-PS-WC) and bag-cultured Ganoderma lucidum polysaccharides (GL-PS-BC) on the proliferation activities of murine spleen lymphocytes in vitro, and investigated whether GL-PS-BC could be substituted for GL-PS-WC. Mixed lymphocyte culture (MLC) reaction, lymphocyte proliferation induced by concanavalin A (Con A, 1μg· mL<'-1>) or lipopolysaccharide (LPS, 5μg·mL<'-1>), MLC reactions inhibited by immunosuppressive drugs, Cyclosporine A (Cs A, 0.1μg·mL<'-1>), Mitomycin C (Mit C, 0.1μg·mL<'-1>), or antitumor drug, Etoposide (VP-16, 0.1μg·mL<'-1>), were detected in the presence or absence of GL-PS-WC and GL-PS-BC at a concentration range of 0.2～12.8μg·mL<'-1>. Two kinds of polysaccharides were shown to promote MLC at the range of 0.2～ 12.8μg·mL<'-1>, increase lymphocyte proliferation induced by Con A or LPS and antagonize the inhibitory effects of CsA, Mit C or VP- 16 on MLC. No significant difference was observed between these two kinds of polysaccharides at selected concentrations. GL-PS- WC and GL-PS-BC showed similar effects on the proliferation activities of murine spleen lymphocytes in vitro.