一株蜂房哈夫尼亚菌的鉴定和产胺能力分析

摘要

A biogenic amine-producing strain named as C10 was isolated from chilled chicken in our laboratory. Its effect on the safety of chilled chicken was evaluated during storage. The strain was identified according to its physiological and biochemi- cal characteristics and 16 rDNA sequence. At the same time, its ability to produce biogenic amine during culture in decarboxylase medium at 4 ℃ for 3 days was analyzed by thin layer chromatography （TLC） and high performance liquid chromatography （HPLC）. The strain C10 was identified as Hafnia alvei. Consistent results were obtained using TLC and HPLC, which showed that C 10 could produce cadaverine, putrescine and histamine at levels of 12.28, 5.23 mg/L and 8.35 mg/L, respectively. In conclusion, Hafnia alvei is a potential hazard to the safety of chicken during refrigeration. In addition, TLC is proved to be a rapid, economical and reliable method for detecting BAs in meats.%考察本实验室从冷藏鸡肉中分离出的一株产生物胺细菌C10的产胺能力，评估其对冷藏鸡肉的食品安全的影响，根据生理生化特性及16S rRNA基因序列对其进行鉴定，并用薄层层析法（TLC）和高效液相色谱法（HPLC）测定其在4℃脱羧酶液体培养基中产生物胺能力。结1果表明，菌株C10为蜂房哈夫尼亚菌（Hafnia alvei），TLC和HPLC检测结果一致，说明C10能产生尸胺（12．28mg／L）、腐胺（5．23mg／L）、组胺（8．35mg／L），因此其会对冷藏鸡肉的食品安全造成潜在风险。同时，TLC与HPLC检测法比较表明，TLC可作为一种经济、快捷、可靠的生物胺筛查方法。