RECOMBINANT PLASMIDE DNA P P1 DIRECTED SYNTHESIS OF FUSED PROTEIN SHOWING ANTIGEN PROPERTIES OF HUMAN IMMUNODEFICIENCY VIRUS I, METHOD OF ITS CONSTRUCTING AND A STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF FUSED PROTEIN SHOWING ANTIGEN PROPERTIES OF HUMAN IMMUNODEFICIENCY VIRUS I

摘要

Usage: Genetic Engineering and Biotechnology. SUMMARY OF THE INVENTION: pVR290 inserted into the vector Hincll-EcoRI fragment of pol region of HIV1 genome encoding a reverse transcriptase and a basin part endonuclease-integrase derived from rVNYu plasmid containing a DNA copy of the viral genome. Plasmid determines ampicillin resistance nekonyugativna. Using the present invention allows to obtain 1 liter of cell suspension 45-65 mg fusion protein comprising the specific sequence of the polypeptide encoded by the pol region of HIV1 genome.