RECOMBINANT PLASMIDE DNA P P1 DIRECTED SYNTHESIS OF FUSED PROTEIN SHOWING ANTIGEN PROPERTIES OF HUMAN IMMUNODEFICIENCY VIRUS I, METHOD OF ITS CONSTRUCTING AND A STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF FUSED PROTEIN SHOWING ANTIGEN PROPERTIES OF HUMAN IMMUNODEFICIENCY VIRUS I

摘要

use: u0433u0435u043du0435u0442u0438u0447u0435u0441u043au0430u00a0 u0438u043du0436u0435u043du0435u0440u0438u00a0 and u0431u0438u043eu0442u0435u0445u043du043eu043bu043eu0433u0438u00a0. the essence of the u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0: vector pVR290 u0432u0441u0442u0440u0430u0438u0432u0430u044eu0442 Hincll - EcoRI pol genome fragment of hiv 1, u043au043eu0434u0438u0440u0443u044eu0449u0435u0433u043e the u0442u0440u0430u043du0441u043au0440u0438u043f - the pelvis and part of the endonuclease is u0438u043du0442u0435u0433u0440u0430u0437u044b, from x of the plasmid u0440u0432u043du044e containing dna copy of the viral genome. u043fu043bu0430u0437u043cu0438u0434u0430 u043eu043fu0440u0435u0434u0435u043bu00a0u0435u0442 resistance to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443, u043du0435u043au043eu043du044cu044eu0433u0430u0442u0438u0432u043du0430.the use of this u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u043fu043eu0437u0432u043eu043bu00a0u0435u0442 receive from 1 l cell suspension of 45 to 65 mg of a hybrid protein containing the specific sequence u043fu043eu043bu0438u043fu0435u043fu0442u0438 yes, encoded pol region of hiv 1.