首页> 中文期刊> 《食品科学 》 >回流提取长白楤木嫩芽皂苷、黄酮工艺研究

回流提取长白楤木嫩芽皂苷、黄酮工艺研究

             

摘要

On the basis of single factor tests, response surface methodology based on Box-Behnken design principle was used to optimize the process parameters of refluxing extraction for saponins and flavonoids from Aralia continentalis, respectively. Results showed that the optimal extraction conditions for saponins were ethanol concentration of 80%, material-liquid ratio of 1:65 (g/mL), extraction time of 2 h and extraction temperature of 94.1℃; the optimal extraction conditions for flavonoids were ethanol concentration of 60%, material-liquid ratio of 1:60, extraction time of 4 h and extraction temperature of 100℃. The yields of saponins and flavonoids were 74.65 mg/g and 19.86 rag/g, respectively, which were consistent with the predicted values of 70.25 mg/g and 20.56 mg/g.%根据中心组合(Box-Behnken)试验设计原理采用响应面分析法,在单因素试验基础上,分别对回流法提取长白楤木中的皂苷与黄酮的工艺参数进行优化。结果表明:回流法提取长白楤木中皂苷的最优工艺参数组合为乙醇体积分数80%、料液比1:65.3(g/mL)、时间2h、温度94.1℃,预测皂苷最大提取量为74.65mg/g,实际测得皂苷最大提取量为70.25mg/g;回流法提取长白楤木中黄酮的最优工艺参数组合为乙醇体积分数60%、料液比1:60(g/mL)、时间4h、温度100℃,预测最大提取量为20.56mg/g,实际测得最大提取量为19.86mg/g。

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