Phenolic compounds from tea seed oil were extracted with 60% methanol and purified by sequential chromatography on silica gel and Sephadex LH-20 columns. The composition of purified phenolics was determined by RP-HPLC-DAD. Phenolic compounds with antiproliferative effect on HepG2 cells were identified by principal component analysis. The results showed that as one of the three fractions separated by Sephadex LH-20, Fr1 contained 5 phenolic compounds including catechins (accounting for 62.60% of the total amount) and flavonoids. Fr2 mainly included phenolic acids and flavonoids; flavonoids represented 78.70% of Fr3. IC50values of Fr1, Fr2, and Fr3 for the proliferation inhibition of HepG2 cells were 1.76%, 16.24%, and 12.35%, respectively. Principal component analysis showed that rutin and catechin were the major constituents in tea seed oil that had a significantly strong ability to inhibit cell viability.%以茶叶籽油为材料,采用60%甲醇溶液提取酚类化合物,经硅胶柱层析后,选取Sephadex LH-20柱对酚类化合物进行分离纯化,并采用反相高效液相色谱-二极管阵列检测技术分析纯化后酚类化合物的组分.以HepG2细胞为供试细胞,结合主成分分析,初步确定茶叶籽油中具有抑制癌细胞活性的酚类化合物.结果表明,Sephadex LH-20柱分离的3个组分中,Fr1组分所含5种化合物主要为儿茶素类或黄酮类,其中儿茶素类占62.60%;Fr2组分主要含酚酸类及黄酮类;Fr3组分中黄酮类占78.70%.Fr1、Fr2、Fr3组分抑制HepG2细胞增殖的IC50值分别为1.76%、16.24%及12.35%.对Fr1、Fr2、Fr3组分中酚类化合物及各组分IC50值进行主成分分析,表明茶叶籽油中儿茶素、芦丁为主要HepG2细胞增殖活性抑制成分.
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