5株樱桃果实采后病原真菌分离及rDNA ITS区序列分析

摘要

从采后贮藏过程中自然发病的“拉宾斯”、“红灯”樱桃果实中分离到5株丝状病原真菌319#、323#、367#、369#和370#.对分离得到的菌株纯化、回接、再分离纯化,并观察菌落形态和个体形态.提取5株病原真菌DNA,PCR扩增rDNA ITS区序列后测序,分析测序结果并构建进化树.综合形态学特征和rDNA ITS区序列鉴定分析结果,得到菌株319#为匍枝根霉(Rhizopus stolonifer)、菌株323#为苹果果腐病菌(Diaporthe perniciosa)、菌株367#为黑附球菌(Epicoccum nigrum)、菌株369#为核果褐腐病菌(Monilinia laxa)和菌株370#为圆孤青霉菌(Penicillium cyclopium),其中D.perniciosa、Enigrum和P.cyclopium为在樱桃果实上首次分离到的病原真菌.%Five fungal strains isolated from naturally infected cherry fruit were purified,re-inoculated and identified as pathogens of cherry fruits.The results of morphological characterization and ribosomal DNA internal transcribed spacer (rDNA-ITS) analysis showed that strain 319#,323#,367#,369# and 370# were identified as Rhizopus stolonifer,Diaporthe perniciosa,Epicoccum nigrum,Monilinia laxa and Penicillium cyclopium,respectively.In this study,D.perniciosa,E.nigrum and P.cyclopium were isolated from cherry fruits for the first time.