具有菲环骨架的高分子载体固定淀粉酶交联聚集体

摘要

Amylase was precipitated with 80％ isopropanol followed by cross-linking with glutaraldehyde to obtain crosslinked amylase aggregates (CLEAs-A).Then CLEAs-A was covalently immobilized on a macroporous polymer carrier containing a phenanthrene skeleton to obtain immobilized CLEAs-A.Herein,various process parameters were systematically evaluated.Moreover,the enzymatic properties and physical structure of immobilized CLEAs-A were investigated.The thermal and storage stability were improved remarkably as compared with those of the free amylase.After seventh repeated use,the activity recovery of immobilized CLEAs-A was still as high as 63.29％.Furthermore,scanning electron microscopy and porosity measurements indicated the surface and pore structure of immobilized CLEAs-A.The proposed immobilization strategy would provide a general approach for preparing immobilized enzymes with robust and high bio-catalytic properties for use in industrial production.%用80％异丙醇溶液将淀粉酶沉淀聚集,随后用戊二醛交联制备交联淀粉酶聚集体(cross-linked amylaseaggregates,CLEAs-A),最后将CLEAs-A共价固定在具有大孔的菲环骨架的载体上,制各固定化CLEAs-A.探讨各种优化条件,研究固定化CLEAs-A的酶学性质,结果显示固定化酶的热稳定性和储存稳定性明显提高,重复反应7批次后,相对活性仍保留63.29％.此外,扫描电子显微镜和孔径测量展现了固定化CLEAs-A的表面和孔径结构.此法可作为一种通用方法,制备出工业生产中所需要的具有高生物催化性能的固定化酶.