The anthocyanins from leaves of the tea cultivar ‘Zijuan’ were separated and purified by sequential column chromatography using MCI gel CHP 20P resin (75-150 μm) and SephadexTM LH-20,through gradient elution with 5％ acidmethanol and 5％ acid-water.A total of 6 anthocyanins were separated from the purified extract,which were analyzed by high performance liquid chromatography (HPLC),thin layer chromatography (TLC) and HPLC-electrospray ionizationtandem mass spectrometry (HPLC-ESI-MS/MS).The results showed that the anthocyanins were identified as delphinidin 3-O-β-D-galactoside,cyanidin 3-O-β-D-galactoside,delphinidin 3-O-β-D-(6-(Z)-p-coumaroyl)galactopyranoside,cyanidin 3-O-β-D-(6-(Z)-p-coumaroyl)galactopyranoside,delphinidin 3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside and cyanidin 3-O-β-D-(6-(E)-p-coumaroyl)galactop yranoside.%依次以MCI gel CPH 20P (75～150 μm)树脂和SephadexTM LH-20葡聚糖凝胶为层析柱填料,对‘紫娟’茶花色苷进行分离纯化,采用5％乙酸-甲醇溶液和5％乙酸溶液对花色苷提取液梯度洗脱,得到6种花色苷组分.采用薄层层析、高效液相色谱及高效液相色谱-电喷雾-串联质谱对‘紫娟’茶花色苷组成成分进行研究.结果表明:从‘紫娟’茶鲜叶中分离出的花色苷为飞燕草素-3-O-半乳糖苷、矢车菊素-3-O-半乳糖苷、飞燕草素-3-O-(6-(Z)对香豆酸)吡喃半乳糖苷、矢车菊素-3-O-(6-(Z)对香豆酸)吡喃半乳糖苷、飞燕草素-3-O-(6-(E)对香豆酸)吡喃半乳糖苷、矢车菊素-3-O-(6-(E)对香豆酸)吡喃半乳糖苷.
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