原花青素B2代谢产物在小鼠体内组织的分布

摘要

为了探究原花青素B2在小鼠体内主要代谢产物形式及其组织分布.采用高效液相色谱-二级阵列-电喷雾-离子阱-飞行时间质谱技术测定分析原花青素B2灌胃小鼠的血浆、肝、心、脾、肺、肾、脑、小肠样品中代谢产物.结果表明,原花青素B2在小鼠体内结合代谢产物形式主要包括:甲基化(8种)、磺酸化(7种)、水合(5种)、羟基化(1种)、葡糖苷酸化(1种);在血浆、肝、小肠、肾、肺、心、脑和脾中检出的原花青素B2代谢产物的种类分别为6、8、8、6、3、6、2、2种,且发现3种单体表儿茶素及其代谢产物.原花青素B2结合型代谢产物多样,在小鼠体内组织分布范围广,甲基化、磺酸化和水合代谢是主要的结合型产物形式,肝脏是原花青素B2结合产物(Mr＞577.13)形成的主要器官,黄烷键(4β→8)的断裂产物是原花青素B2重要的吸收形式之一.%In the present study,the metabolite profiles of procyanidin B2 in the plasma,liver,heart,spleen,lung,kidney,brain and small intestine of mice were qualitatively evaluated by high performance liquid chromatography with diode array detector coupled with electrospray ionization ion trap time-of-flight multistage mass spectrometry (HPLC-DAD-ESI-IT-TOF-MSn).The results showed that the major conjugated metabolites included methylation metabolites (8),sulfonation metabolites (7),hydration metabolites (5) and hydroxylation metabolites (1),glucuronidation metabolite (1).Procyanidin B2 was metabolized into 6,8,8,6,3,6,2 and 2 forms in plasma,liver,small intestine,kidney,lung,heart,brain and spleen,respectively;three forms of identified metabolites were found with cleavage of interflavanic bond.The conjugated metabolites of procyanidin B2 were diverse and distributed widely in various tissues of mice.Methylation metabolites,sulfonation metabolites and hydration metabolites were dominant,and the liver was the main organ for the conjugated metabolites of procyanidin B2 according to product formation (Mr ＞ 577.13).The cleavage of interflavanic bond reaction was an important way that procyanidin B2 is absorbed.