4种防腐剂对副溶血弧菌生物膜形成的抑制作用

摘要

在确定4种防腐剂壳聚糖、山梨酸钾、脱氢乙酸钠和ε-聚赖氨酸对副溶血弧菌(Vibro parahaemolyticus)的最小抑菌浓度(minimum inhibitory concentration,MIC)的基础上,研究其对副溶血弧菌生物膜的抑制作用.采用结晶紫染色法测生物膜形成量,XTT法测生物膜代谢活性,硫酸-苯酚法测定生物膜中胞外多糖的分泌量.结果表明,壳聚糖的MIC最小,为1.25 mg/mL.4种防腐剂在MIC以及亚抑菌浓度条件下对副溶血弧菌生物膜均有明显的抑制作用,不仅抑制生物膜的形成,而且能显著降低细菌的代谢活性,减少胞外多糖的分泌,其中壳聚糖对副溶血弧菌生物膜的抑制作用最强.%In this study,we analyzed the minimum inhibitory concentrations (MICs) of four kinds of food preservatives (chitosan,potassium sorbate,sodium dehydroacetate and ε-polylysine) for Vibro parahaemolyticus,and we then investigated their inhibitory effects on biofilm formation by V.parahaemolyticus.The crystal violet straining method was conducted to determine the amount of biofilm,and XTT reduction assay and phenol-sufuric acid method were used to detect the changes of metabolic activities and extracellular polysaccharide of biofilm,respectively.The results showed that the MIC of chitosan was the lowest,which was 1.25 mg/mL.At both MIC and sub-MIC,these four food preservatives could not only demonstrate a remarkable inhibitory effect on biofilm formation by V.parahaemolyticus,but also reduce the metabolic activity and the secretion of extracellular polysaccharides of cells in biofilms.Among these preservatives,the inhibitory effect of chitosan was the strongest.