利用微波辅助提取枳实总黄酮,并对提取参数进行优化.以过40目筛的脱脂枳实粉为原料,探讨乙醇浓度、液固比、提取时间、微波功率等因素对总黄酮得率及干膏率的影响.在单因素试验基础上,对乙醇浓度、液固比和提取时间3个因素进行Box-Behnken设计,以总黄酮含量、干膏率为响应值,利用响应面分析法对提取参数进行优化.在乙醇浓度70%、液固比40:1(mL/g)、微波功率385W(中火)下提取1.5min,总黄酮含量达到41.39%,干膏率49.00%.响应面法优化枳实黄酮的提取比优化前单因素试验结果有很大的提高.%Studies were made on extracted flavnonids from Frutus aurantii immatures by microwave-assisted extraction, and optimized the extracted factors. The effect of different ethanol concentrations, ratio of solvent to material, extraction time and microwave frequency on yield of total flavonoids and dry extraction ratio were discussed. The Box-Behnken design was applied to fit a response surface function, which based on the one-variable-at-time design. Through the function we can get the optimum extracting conditions, the optimal levels for obtaining 41.39% total flavonoids and 49.0% dry extraction ratio were as follows: The ethanol concentration is 70%, the ratio of solvent to material is 40:1 (mL:g), the microwave frequency is 385 W, and The extraction time is 1.5 min. The Box-Behnken design can be used on the extraction of flavnonids from Frutus aurantii immatures, and the result is better than that in the one-variable-at-time design.
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