干燥蒜粉中苯甲酸检测的假阳性分析

摘要

以GB/T5009.29-2003液相色谱条件检测干燥蒜粉样品里的苯甲酸时,出现引起误判的干扰性物质,当HPLC方法经过适当的调整(梯度洗脱条件:0min,5%B,流速0.7mL/min;5min,5%B,流速0.7mL/min;7min,7%B,流速1mL/min;12min,5%B,流速1mL/min;15min,5%B,流速1 mL/min;30min,5%B,流速1mL/min,B相为甲醇),可避免这个问题.%There are some interfering matter as detecting benzoic acid in dry garlic powder by the method of GB/T 5009.29-2003. As adjusting the method of HPLC properly (the condition of gradient elution: 0 min, 5% B, velocity of flow: 0.7mL/min; 5 min, 5 %B, velocity of flow: 0.7 mL/min; 7 min, 7 %B, velocity of flow: 1 mL/ min; 12 min,5 %B, velocity of flow: 1mL/min; 15 min,5 %B, velocity of flow: 1 mL/min;30 min,5 %B,velocity of flow: 1 mL/min B:ethanol), the problem can be avoided.