探讨酶解法提取近江牡蛎糖胺聚糖(SG1)的务件,结果表明:先用枯草杆菌中性蛋白酶1.0%(质量分数),pH7.0,50℃酶解5 h,再加胰蛋白酶1.0%(质量分数),pH7.8,50℃酶解5 h效果最佳.粗提物SG1的得率为2.40%,总糖胺聚糖含量为13.6%,总糖含量为61.3%.采用四氮唑蓝还原法(MTT法)研究粗提物SG1对人宫颈癌细胞(Hela细胞)的体外抗肿瘤活性,结果显示:500μg/mL剂量组与600μg/mL剂量组48h时的抑制率分别为43.1%(P<0.05),55.8%(P<0.05),近江牡蛎糖胺聚糖粗提物SG1具有较明显的抗肿瘤活性,存在一定的量效关系.%The extraction conditions of crude glycosaminoglycan (SG1) by enzymatic hydrolysis from Crassostrearivularis Crould were studied in this paper. The results showed that the optimum conditions were:slurry of Crassostrearivularis Crould adding neutral protease of Bacillus subtilis, enzyme dosage 1.0 %(w/v), pH 7.0, temperature 50℃, enzymatic hydrolysis time 5 h, then adding trypsin enzyme with dosage 1.0 %(w/v), pH 7.8, temperature 50℃ and time 5 h. The extraction ratio of the crude glyeosaminoglycan (SG1), the total content of glycosaminoglycan and its total sugar respectively were 2.40 %, 13.6 % and 61.3 %. Then the anti-tumor activity in vitro to cell Hela of crude glycosaminoglycan by MTT assay was investgated. It turned out that the inhibition rates of the dose groups (500 μg/mL and 600 μg/mL) respectively were 43.1%(P<0.05)and 55.8% (P<0.05).The crude glycosaminoglycan(SG1) from Crassostrearivularis Crould had marked anti-tumor function which was concentration-dependent.
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