金属离子对桑黄菌丝体及胞外多糖含量的影响

摘要

筛选出促进桑黄菌丝体生物量和胞外多糖的金属离子。通过测定平板上菌丝体生长速度，液体发酵的菌丝体干重和胞外多糖含量，确定金属离子对桑黄生长的影响。在固体平板培养中，Mg2+、Fe2+、Ca2+对桑黄的生长速度具有明显的促进作用，Na+对菌丝生长速度无明显的促进作用，而在液体摇瓶发酵中，1、3 mg/mL Mg2+，0.9 mg/mL Fe2+，3、5、7 mg/mL Ca2+以及3 mg/mL Na+增加菌丝体干重量，较空白对照差异显著，是空白对照的5倍~8倍。在3 mg/mL K+和Na+，7 mg/mL Ca2+，0.7 mg/mL Fe2+，0.1 mg/mL Zn2+的培养基中桑黄分泌的胞外粗多糖含量分别为0.4760、0.7105、0.5200、0.4516和0.6820 g/100 mL，较空白对照显著差异。一定浓度的金属离子对桑黄菌丝体的生长和胞外多糖分泌有一定的促进作用。%To screen the different mineral ions for promoting the growth of mycelia and crude extracellular polysaccharide of hellinus igniarius. Impact of metal ions on the Santo effect on the growth of P. linteus was identified by determination of growth rate of Mycelia in solid surface culture method in medium with different metal ions, mycelia dry weight and crude extracellular polysaccharide obtained in shake -flsak fermentation. The results show that the medium with Mg2+,Fe2+,Ca2+could obviously promote the growth of the mycelia of P. inteus on plate cultivation, while the Na+has no obvious role.In medium with 1 mg/mL and 3 mg/mL Mg2+, 0.9 mg/mL Fe2+, 3,5 mg/mL and 7mg/mL Ca2+,and 3 mg/mLNa+,the dry weight of mycelia were 5-8 folds than that of control medium,In medium with 3 mg/mL K+and Na+, 7mg/mL Ca2+,0.7 mg/mL Fe2+and 0.1 mg/mL Zn2+, the contains of extracellular polysaccharide secreted by P.inteus were 0.476 0, 0.710 5, 0.520 0, 0.451 6 g/100 mL and 0.6820 g/100 mL,respecrively, the difference was significient compared with control medium. The different metal ions had the role of promoting the growth of the mycelia of P. inteus and the secretion extracellular polysaccharide.