小分子半抗原直接包被ELISA用于虾肉氯霉素检测

摘要

The novel ELISA was developed for monitoring chloramphenicol remains in food by the directly attaching small molecule hapten with amino groups to the microtiter plate surface. chloramphenicol with amino-groups was directly coated on the surface of microtiter plates by treating with glutaraldehyde solution , and biotin-streptavidin system was employed to improve the sensitivity of immunoassay. The sensitivity of the new ELISA was 12.9 ng/mL, CV%was 1.8%-4.5%. The novel amination small molecule hapten coupled directly ELISA has many advantages, such as high sensitivity, less interference, simple operation and so on.%探讨新型氨基化小分子半抗原直接包被ELISA方法检测食品中氯霉素残留的可行性。采用氨基化氯霉素直接包被在经戊二醛活化后的酶标板表面，并通过生物素-链霉亲和素放大系统进一步提高该检测方法的灵敏度。结果表明，氨基化小分子半抗原直接包被ELISA的灵敏度和最低检出限分别为12.9 ng/mL和0.7 ng/mL，CV%为1.8%~4.5%。新型氨基化小分子半抗原直接偶联ELISA具有灵敏度高，干扰少，检测步骤简便、操作安全，测定结果较准确可靠。