CRUDE NATIVE HAPTEN-BASED INDIRECT ELISA ASSAY KIT AND LYOPHILISED CONTROLS FOR THE CONFIRMATORY DIAGNOSIS OF BOVINE BRUCELLOSIS IN BLOOD SERUM AND MILK BY ANIMAL AND TANK

摘要

A diagnostic kit for a confirmatory assay using the indirect ELISA method that measures the levels of anti-native hapten antibodies produced in an actual infection, thereby preventing large financial losses to the farm, by discerning false-positives that present anti-LPS antibodies due to cross-reactions with enterobacteria and post-vaccine antibodies for the diagnosis of bovine brucellosis in blood serum and milk by animal and tank, characterised by using the crude native hapten antigen, extracted from the strain B. melitensis 16Μ, with no purification treatment and an effective adherence capacity, which is used to sensitise or antigenise plates at a known concentration (1 μg per well), wherein positive and negative controls subjected to the lyophilisation method to ensure their preservation, thereby preventing the contamination and degradation of the antibodies present and ensuring the stability of the optical densities in said controls for correct interpretation of the results of an indirect ELISA, are taken as reference. The lyophilisation method for controls that may be used in other diagnostic methods is also presented.