毛细管电泳涂层研究及食品检测中的应用

摘要

Capillary electrophoresis coatings were burned in order to separate basic proteins. The researchers had going in for improve separating property with new materials and methods. Coating the internal surface area of capillary column with functional materials becomes the most efficient and commonly used approach until now.In recent years,with the rapid developments of the capillary electrophoresis (CE) online coupling techniques with mass spectrometry (MS) and laser-induced fluorescence (LIF) spectrometry,CE has been widely used in the areas of life science,environment protection and food detection. In the paper,the latest progress about capillary coatings is reviewed based on the different materials and preparation mechanisms , the application in food detection is also discussed.%毛细管电泳涂层技术因为分离碱性蛋白的需求而诞生，研究者致力于采用不同的材料和方法提高毛细管电泳的分离性能。目前，对毛细管内壁进行涂层改性依然是提高毛细管电泳的分离效果和重现性，抑制分析物与毛细管内壁间吸附作用的最有效、最常用的方法。随着毛细管电泳（CE）与质谱（MS），激光诱导荧光检测（LIF）等联用技术的发展，毛细管电泳涂层在生命科学技术、生物医药工程、环境卫生保护、食品质量检测等领域的广泛应用。本文根据涂层材料的种类和制备机理，对近年来毛细管电泳涂层技术的特点、发展和在食品检测领域的应用状况进行了综述。