壶瓶枣提取物抗氧化性研究

摘要

以水和乙醇为提取剂，运用超声强化方法提取了壶瓶枣中水溶性及醇溶性成份。采用DPPH·、HO·、O2-·、还原能力及金属螯合能力方法研究了提取物的抗氧化活性，并对提取物中的总黄酮含量进行了测定。结果表明：两种提取物均具有一定的抗氧化活性，水提取物的抗氧化能力为：O2-·（IC501.80μg/mL）＞DPPH·（IC504.13μg/mL）＞HO·（IC5061.04μg/mL），醇提取物的抗氧化能力为：DPPH·（IC500.58μg/mL）＞O2-·（IC501.70μg/mL）＞HO·（IC5060.03μg/mL），醇提物的金属螯合能力和还原能力均高于水提物。提取物的抗氧化活性与其总黄酮含量具有一定的相关性。%Ultrasound-assisted extraction was used to extract activated components from Zizyphus jujube cv. Huping using water and ethanol as extracting agents. DPPH·, HO·, O2-·, reducing powers and ferrous iron chelating capacities were carried out to evaluate the antioxidant potential of both of the extracts. The constant of total flavonoid of both of extracts was determined. The result showed that both extracts exhibit good antioxidant;The order for the free radical scavenging activities of the water extract was O 2-· (IC50 1.80μg/mL), DPPH·(IC50 4.13μg/mL) and HO·(IC50 61.04μg/mL), and that of ethanol extract were DPPH (IC50 0.58μg/mL), O2-·(IC50 1.70μg/mL), and HO·(IC50 60.03μg/mL). The reducing powers and ferrous iron chelating capacities of ethanol extract was better than that of water extract. In addition , there was a positive correlation between the antioxidant and the total flavonoid content of the extracts.