槟榔花中酚类物质对HSF细胞氧化损伤的保护作用

摘要

为了探讨槟榔花中3种主要酚类物质(表儿茶素、没食子酸、香豆酸)对人皮肤成纤维细胞(HSF)氧化损伤的保护作用,以H2O2(150μmol/L)处理HSF细胞24 h,建立氧化损伤模型,MTT法测定细胞存活率,分光光度法检测细胞内总抗氧化能力和线粒体膜肿胀度,DCFH-DA染色法测定细胞内活性氧的含量. 结果表明表儿茶素和没食子酸均能改善H2O2引起的HSF细胞的氧化损伤,而香豆酸却没有保护作用.与H2O2模型组相比,表儿茶素和没食子酸均能提高氧化损伤细胞的存活率,并将细胞内总抗氧化能力提高了4.73%~31.66%,细胞内活性氧和线粒体膜肿胀度也明显降低.表明槟榔花中2种主要的酚类物质表儿茶素和没食子酸对HSF细胞氧化损伤具有保护作用.%In order to understand the protective effects of 3 kinds of phenolic compounds (epicatechin, gallic acid, coumaric acid) in Areca inflorescence on oxidative damage in human skin fibroblasts cells (HSF), HSF cells were treated with 150μmol/L H2O2 for 24 h and the oxidative damage model was established. Cell viability was measured by MTT method, the total antioxidant capacity and mitochondria membrane swelling degree were detected by spectrophotometry, intracellular reactive oxygen species (ROS)accumulation was measured by DCFH-DA staining method. The results showed that epicatechin and gallic acid could improve the oxidative damage on HSF cells induced by H2O2, whereas coumaric acid had no protective effect. Compared with the H2O2 model group, epicatechin and gallic acid can improve the survival rate of HSF cells, the total antioxidant capacity was increased by 4.73 %-31.66 %, intracellular reactive oxygen species and the mitochondrial swelling decreased. As a results, the two main kinds of phenolic compounds (epicatechin and gallic acid ) in Areca inflorescence have protective effects on oxidative damage in HSF cells.