Protective effect of rutin against high glucose-induced oxidative damage in PC12 cells

摘要

Objective: This study evaluated the protective effect of rutin on high glucose-induced damage in PC12 cells. Methods: Viability of PC12 cells induced by high glucose was detected by MTT assay and cell death by lactate dehydrogenase release. Nucleic acid status in the cell was observed by Hoechst 33342 staining. NO and NOS were measured using detection assay kits. Results: PC12 cells were pretreated with various concentrations of rutin for 1 h and then coped with rutin and D-glucose for 48 h. Rutin protected PC12 cells against high glucose damage, which was determined by cell viability and apoptosis as evaluated by Hoechst 33342 staining assay. Rutin inhibited increased NOS activity in a concentration-dependent manner. Increased NO level was significantly reduced by rutin. Conclusions: Findings revealed the protective effect of rutin against high glucose-induced oxidative damage in PC12 cells. Results suggest that rutin could be a potentially natural compound for diabetic neuropathy treatment.