响应面法优化“酶法-超声波”连续提取核桃青皮多酚

摘要

为了优化核桃青皮中多酚类物质的“酶法-超声波”连续提取工艺，在单因素试验基础上，通过Design Expert 8.05软件进行响应面设计与优化，得到了预测数学模型。结果表明，酶法提取最佳工艺条件为：采用纤维素酶，酶解温度55.00℃、酶解pH 5.00、酶解时间75.00 min、酶添加量15.00 U/mL、液料比30∶1 mL/g。酶解后继续采用超声波辅助二次提取，以50%“乙醇-水”为提取液，超声时间45 min、超声温度50℃、超声功率100 W，此条件下多酚提取率为61.36%。%In order to optimize the enzymatic-ultrasonic continuous extraction processes of polyphenols from walnut green peels,the optimum extraction technology and mathematical regression model were established by response surface methodology on the basis of single-factor test. The results showed that the optimal extraction conditions were extraction temperature of 55℃, extraction time of 75.00 min, extraction pH of 5.00, enzyme dosage of 15.00 U/mL, ratio of liquid to solid of 30∶1 mL/g. And the ultrasonic-assisted extraction was operated after enzymatic extraction for 45 min at the power of 100 W by 50%ethanol solution as extractant. Under these conditions, the maximum extraction yield of polyphenols from walnut green peels was 61.36%.