紫娟茶中花青素提取物的抗氧化性研究

摘要

Anthocyanins from Zi juan tea were exteacted by using acid-containing methanol as solvent and patitioned with ether and chloroform. The extract of anthocyanins was purified by Amberlite XAD-7HP chro-matograpHy. The antioxidant and free radical scavenging capacity of anthocyanin extracts .were evaluated by DPPH method, ABTS method and FRAP method. The results showed that the ability of scavenging DPPH:an-thocyanins extracts>BHA>BHT;the ABTS radical scavenging activities and the FRAP antioxidant:BHA>an-thocyanins extracs>BHT.%紫娟茶由含酸甲醇提取后，经过乙醚、氯仿的液液分配以及Amberlite XAD-7HP树脂的吸附洗脱，得到花青素粗提物。针对花青素提取物，采用DPPH法、ABTS法、FRAP法研究探讨了其抗氧化性及清除自由基的能力。试验结果显示，捕捉DPPH自由基的能力：紫娟茶中花青素提取物＞BHA＞BHT，清除ABTS+阳离子自由基的能力和FRAP抗氧化能力都是：BHA＞紫娟茶中花青素提取物＞BHT。