An accurate and sensitive method for the detection of amantadine (AM) residues in animal-derived food products was developed by combining solid-phase extraction with high performance liquid chromatogra-phy-tandem mass spectrometry(SPE-HPLC-MS/MS). The Oasis MCX solid-phase extraction column was used for complex matrix purification and Agilent SB-C18 column(2.1 mm ×150 mm, 3.5μm) was employed for sep-aration in liquid chromatography using a mobile phase consisting of 0.1%formic acid and acetonitrile (80:20, volume ratio)with the flow rate 0.2 mL/min. The electrospray ionization (ESI) source in the positive mode and the multiple-reaction monitoring (MRM) mode were used for the quantitative analysis with external standard method. The results showed that the calibration curves were in good linearity for the AM ranged from 0.1μg/L to 100μg/L with the correlation coefficients(R2)>0.999.The limits of detection(LODs) and the limits of quantifi-cation (LOQs) of this method were 1.0μg/kg and 3.0μg/kg respectively.The average recovery rates were from 83.6%to 94.2%with the good relative standard deviations (RSDs, RSD0.999.该方法检出限(Limit of detection,LOD)为1.0μg/kg,定量限(Limit of quantitation,LOQ)为3.0μg/kg,对3个添加浓度(30.0、60.0、90.0μg/kg)下的鸡胸,鸡肝,鸡蛋,猪肉,羊肉5种样品中金刚烷胺残留的检测具有较高的准确度(回收率在83.6%~94.2%之间)和重现性(RSD<4.0%,n=3).
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