芒果核多酚纯化物成分分析及其抗氧化研究

摘要

采用30%、50%、70%、90%的乙醇回流提取芒果核多酚物质,把提取液浓缩浸膏,依次采用硅胶柱层析法、薄层色谱跟踪检测、红外光谱、核磁共振等方法进行检测分析.结果表明,30%和50%乙醇纯化物中主要成分是鞣酸,70%乙醇纯化物是鞣花酸,90%乙醇纯化物是蓖麻油酸,进一步的纯化单体分别为芥酸以及油酸甘油酯;抗氧化活性试验表明,芒果核多酚及其纯化物对羟基自由基(·OH)、DPPH自由基(DPPH·)清除作用明显,说明芒果核多酚具有很好的抗氧化活性,为芒果核的综合开发利用提供重要依据.%Polyphenols of mango kernel seeds were extracted by different concentration of ethanol(30%, 50%, 70%and 90%), and then separated by silica gel column chromatography, thin layer chromatography. The structure of the purified compound products were analyzed by infrared spectrum and nuclear magnetic reso-nance. The main component purified by 30%or 50%ethanol was gallic acid, while 70%ethanol and 90%ethanol were ellagic acid and castor oil acid, respectively. The further purifying and identifying the component purified by 90%were determined to be erucic acid and 1,2-Dioleoy1-rac-glycerol.The results of antioxidation experiments showed that the purified polyphenols from mango kernel seeds had stronger antioxidant activity a-gainst hydroxyl radicals (·OH) and DPPH radicals (DPPH·), which would provide an important basis for comprehensive utilization of mango kernel.