响应面优化生姜多糖提取工艺及其抗氧化活性研究

摘要

The extraction of polysaccharides from ginger was optimizition by Bom-Benhnken desing,a multiple quadratic regression describing the relationship between the parameters such as extraction time,extraction tem-perature,liquid-to-material ratio were established using a 3-factor,3-lever Box-Behnkon design, and the structure of polysaccharides was analysised by FTIR.The results showed ,the optimal extraction condition was obtained as extraction time 2 h 44 min ,extraction temperature71.6℃,liquid-to-material ratio 41:1(mL/g). Under such conditon the yield of polysaccharides from ginger was 3.13%.From some experimengts,we find gin-ger polysaccharides have antioxidant activity .%采用Box-Benhnken法优化生姜多糖的提取工艺参数,分别考察提取时间、提取温度及液固比对多糖得率的影响,采用三因素三水平响应面法进行多糖提取工艺的优化,利用傅立叶红外光谱分析其结构.结果表明,生姜多糖最佳提取时间为2 h 44 min,提取温度为71.6℃,液固比为41:1(mL/g),在此条件下,生姜多糖得率为3.13%.后经抗氧化活性测定,生姜多糖提取物具有一定抗氧化活性.