Ultrasonic-assisted extraction of polysaccharides from Boussingaultia gracilis was optimized, and the antioxidant properties of polysaccharides were determined. On the basis of single factor experiment, the opti-mum conditions of ultrasonic-assisted extraction of polysaccharides were optimized by orthogonal test. Mean-while, the antioxidant activity of polysaccharides from Boussingaultia gracilis was evaluated by hydroxyl, DPPH and superoxide anion free radical scavenging capacity. Results showed that the optimal conditions of polysac-charides extraction as follows:liquid to solid ratio 50:1(mL/g), ultrasonic temperature 50℃, ultrasonic pow-er 350 W, and ultrasonic time 60 min. Under the optimal conditions, the yield of polysaccharides was 6.24%, the average recovery rate of the added sample was 94.56 %. When the polysaccharides concentration was 0.0242 mg/mL, the clearance rate of polysaccharides on·OH,·DPPH and O2-·were 67.59%, 61.01%and 81.46%, respectively, implying such material had a strong antioxidant activity in vitro.%以藤三七为材料,优化超声波辅助提取藤三七多糖的提取工艺,并测定其多糖的抗氧化性.在单因素试验的基础上,通过正交试验优化超声波辅助提取多糖的最佳工艺条件;并通过藤三七多糖对·OH、DPPH·和O2-·的清除来评价抗氧化性.结果表明:藤三七多糖的最佳提取工艺条件为:液料比50:1(mL/g)、超声温度50℃、超声功率350 W,超声时间60 min,在此条件下多糖提取率为6.24%,平均加样回收率为94.56%.多糖质量浓度为0.0242 g/mL时,对·OH、DPPH·和O2-·的清除率可分别为67.59%、61.01%和81.46%,说明藤三七多糖具有较强的体外抗氧化活性.
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