UPLC-MS/MS同时测定肉汤中5种罂粟壳生物碱

摘要

A method for simultaneous determination of 5 opium poppy husk alkaloids in broth sample was de-veloped by ultra performance liquid chromatography-electrospray tandem mass spectrometry. Broth samples were extracted by 0.1 mol/L hydrochloric acid, then concentrated and purified by ProElut PXC solid-phase ex-traction column, after that were eluted by 5%ammonia-methanol.The separation was performed on a Thermo Hypersil Gold(100 mm×2.1 mm×1.9μm)column,and detected by mass spectrometry using electrospray ioniza-tion (ESI) in positive mode under multiple reaction monitoring mode.The recovery rates of the spiked sample was in the range of 71.8%-112.5%and the relative standard deviation (RSD) was between 4.1%to 11.0%(n=6). The detection limits for morphine,codeine,thebaine,papaverine and narcotine were 1.2,0.8,0.4, 0.05 μg/kg and 0.04μg/kg.This method can be successfully applied to the simultaneous detection for 5 opium poppy husk alkaloids in broth.%建立超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定肉汤中5种罂粟壳生物碱含量的方法.肉汤样品用0.1 mol/L盐酸提取,经ProElut PXC固相萃取柱富集净化后,5%氨水-甲醇溶液洗脱.采用Thermo Hypersil Gold色谱柱(100 mm×2.1 mm×1.9μm)分离,ESI正离子扫描,多反应监测模式采集测定.3个浓度添加水平的回收率在71.8%~112.5%之间,相对标准偏差(RSD)为4.1%~11.0%(n=6).吗啡、可待因、蒂巴因、罂粟碱、那可丁的检出限分别为1.2、0.8、0.4、0.05、0.04μg/kg.本方法可用于同时检测肉汤中5种罂粟壳生物碱.