制备铅离子多克隆抗体,并建立间接竞争ELISA检测方法.利用双功能螯合剂ITCBE将Pb2+与载体蛋白KLH和BSA偶联在一起,制备免疫抗原与检测抗原；通过免疫新西兰大耳白免成功制备铅多克隆抗体,建立间接竞争ELISA检测方法,并与ICP- MS检测结果进行比较.标准曲线IC50为3.48 ng/mL,IC15为0.32 ng/mL,与ICP- MS法检测结果的总符合率超过97％.%To prepare polyclonal antibody against heavy metal lead ion and develop an indirect competitive ELISA method. Lead was coupled to vector proteins keyhole limpet hemocyanin( KLH) and bovine serum albumin(BSA) via a bifunctional chelating agent 1TCBE respectively, Pb(D HTCBE-KLH was used as immunogen and Pb( II)-ITCBE-BSA was used as coating antigen. The anti-Pb antibody was obtained after immunized to New Zealand white rabbits and an indirect competitive ELISA method was developed. by which the determination result was compared with that by ICP-MS method. The standard curve was developed with JC50 of 3. 48 ng/mL and IC15 of 0. 32 ng/mL, the total coincidence rate of the developed ELISA method and ICP-MS method was more than 97%.
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