枳椇果梗不同类型酚酸含量及抗氧化活性

摘要

Four different kinds of phenolic acids of Hovenia acerba fruit were prepared by acid or alkaline hydrol- ysis and organic solvent extraction. And they are soluble free phenolic acids （SFPA） , soluble esterified phenolic acids （SEPA） , soluble glycoside phenolic acids （SEPA） and methanol-insoluble ester-bound phenolic acids （ISPA）. The spectral characteristics were studied by scanning Ultraviolet Spectrum in the range of 200 -800 nm. The total phenolic acids content and the antioxidant activity of different kinds of phenolic acids of Hovenia acerba fruit were determined by Folin-Ciocaheu method, and DPPH, ABTS ^＋ , and Fe^3＋ methods, respectively. Results indicated that the crude extraction and four different phenolic acids have different spectral characteristics in ultraviolet band. The total phenolic acids content of ISPA is striking higher than the three kinds of soluble phenolic acids. The order of total phenolic acids content is ISPA 〉 SEPA 〉 SFPA 〉 SGPA. Antioxidant activities of ISPA is also markedly stronger than the three kinds of soluble phenolic acids.%通过酸碱水解，有机溶剂萃取的方法制备枳棋果梗4种不同类型酚酸——游离酚酸（SFPA）、酯型酚酸（SEPA）、糖苷型酚酸（SGPA）、不溶性结合型酚酸（ISPA）。对各部分酚酸进行紫外可见光谱扫描确定其紫外吸收特性；以Folin-Ciocalteu法测定总酚酸含量，采用清除DPPH、ABTS^＋自由基和Fe^3＋还原法对不同类型酚酸体外抗氧化活性进行评价。结果表明：枳楔果梗不同类型酚酸均有特征紫外吸收；不同类型酚酸的总酚含量大小依次为：不溶性结合型酚酸〉酯型酚酸〉游离酚酸〉糖苷型酚酸，不溶性结合型酚酸含量显著高于其他三类酚酸；在3种抗氧化体系中，不溶性结合型酚酸也表现出最强的抗氧化活性。