芥末等8种食物过敏原的多重PCR检测技术

摘要

Eight pairs of primers were selected according to the sequence of Sin a I gene from mustard,ITS（18S~26S） gene from lupine,Jug r 2 gene from walnut,Oleosin gene from hazelnut,Mtd gene from celery,Pru du 1 gene from almond,Avenin gene from oat and 2S albumin mRNA gene from sesame.Two tetraplex PCR assays for simultaneously detection of celery,almond,oat,sesame,mustard,lupine,walnut and hazelnut were established on the basis of conventional PCR.It could be demonstrated to be a highly sensitive,specific method,which can be applied for rapid detection and monitoring of many kinds of food allergens.%依据芥末Sin a I基因、羽扇豆核糖体内转录间隔区（ITS）基因、胡桃Jug r 2基因、榛果Oleosin基因、芹菜Mtd基因、杏仁Pru du 1基因、燕麦Avenin基因和芝麻2S albumin mRNA基因设计的特异性引物序列,在普通PCR方法的基础上,通过2组4重PCR扩增,建立了同时检测芹菜、杏仁、燕麦、芝麻、芥末、羽扇豆、胡桃和榛果等8种食物过敏原的方法。该方法特异性强,灵敏性高,可应用于食品中多种食物过敏原的快速检测和监控。