四川白兔的氨基酸组成分析及营养价值评价

摘要

To analyze the intramuscular amino acid composition in different ages of Sichuan white rabbit and evaluate its nutritional value,the rear leg muscles,longissimus dorsi and abdominal muscles of Sichuan white rabbit were used.The amino acid was analyzed by HC1 hydrolyzed method and determined by HITICHI L-8900 automatic analysis instrument.The results show that Sichuan white rabbit muscle protein consists of at least 17 kinds of amino acids,seven essential amino acids for human.The total amino acids,essential amino acids,and ratio of essential amino acids and total amino,flavor amino acids all increased with ages;and the ratio of essential amino acids and total amino acids of rear leg muscles and longissimus muscles is higher than the recommended value in Food and Agriculture Organization(FAO) / World Health Organization (WHO),so rear leg muscles and longissimus muscles are high-quality protein meats.The amino acid score (AAS),chemical sore (CS),essential amino acid index (EAAI)of Sichuan white rabbit muscle proteins were evaluated.It was found that the majority of the essential amino acids is higher than FAO / WHO recommended standards,but lower than the egg mode of amino acids.The AAS and CS have shown that the first limited amino acid of Sichuan white rabbits is methionine and cysteine acid,and the second limited amino acid is valine.The above results suggest that Sichuan white rabbit has a high nutritional value.%分析了四川白兔不同日龄、不同部位的氨基酸组成,探讨其营养价值,以四川白兔的腿部肌、背最长肌、腹部肌为实验材料,采用盐酸水解法,使用日立L-8900全自动氨基酸分析仪进行测定.结果表明,四川白兔肌肉蛋白质至少由17种氨基酸组成,其中包含7种人体必需氨基酸.各个部位的氨基酸总量、必需氨基酸总量、必需氨基酸与总氨基酸的比值、呈味氨基酸总量总体均随着日龄的增加而增加,腿部肌和背最长肌的必需氨基酸占总氨基酸的比例优于联合国粮农组织(Food and Agriculture Organization,FAO)/世界卫生组织(World Health Organization,WHO)提出的推荐值,属于优质蛋白质.采用氨基酸评分(amino acid score,AAS)、化学评分(chemical sore,cS)、必需氨基酸指数(essential amino acid index,EAAI)对四川白兔肌肉蛋白质的营养价值进行了评价,发现多数必需氨基酸的含量高于FAO/WHO推荐的氨基酸模式含量,低于全鸡蛋模式中氨基酸的含量,但AAS 和CS均显示,四川白兔的第一限制性氨基酸为蛋氨酸和胱氨酸,第二限制性氨基酸为缬氨酸.这些结果表明,四川白兔具有较高的营养价值.