香菇生物碱类物质的毛细管电泳指纹图谱

摘要

采用快速溶剂法醇提香菇中生物碱类物质,进行毛细管电泳色谱分析.毛细管电泳优化条件:背景电解质溶液:25 mmol/L硼砂-10 mmol/L β-环糊精,pH:9.5,运行电压:20 kV,进样条件:20 psi,5 s,温度:25℃,检测波长:214 nm.毛细管电泳色谱方法学考察,其精密度、稳定性和重复性均符合指纹图谱要求,分别建立了福建、湖北、吉林、云南等地的香菇生物碱类成分的毛细管电泳指纹图谱,确定14个共有峰,通过相似度评价系统对10批香菇进行评价,多数香菇样品的相似度在0.8以上,可用于香菇的质量控制.%Accelerated solvent extraction was used in extracting alkaloids from lentinus edodes and its analysis was performed by capillary electrophoresis.The optimized conditions of capillary electrophoresis were:background electrolyte 25 mmol/L borax-10 mmol/L β3-cyclodextrin;pH 9.5;the running voltage 20 kV;the sample size 20 psi × 5 s;the column temperature 25 ℃ and the detection wavelength 214 nm.Capillary electrophoresis methodology analysis showed that the method is precision,stable and reproductive.They were meet with the requirements of fingerprints.The capillary electrophoresis fingerprints of the alkaloids of letinous edodes in different regions were established.We found out 14 common peaks from the fingerprints,and evaluated the similarity of 10 batches of lentinus edodes through professional similarity evaluation system.The results showed that similarity of majority of those lentinus edodes were above 0.8 which indicate that the method can be served as a novel reference to identify and control the quality of lentinus edodes.