Problems Associated with the Liquid Cultivation of Shiitake, 'Lentinula edodes'(Berk.) Pegler

摘要

Different cultivation methods were compared in order to determine the best methodfor the large-scale production of shiitake liquid spawn. Fermenter spawn was the most suitable for large-scale production and adapted well in the supplemented sawdust medium. The drawbacks of liquid spawn are a tendency for degeneration and mutation, after repeated subculturing. Genetic changes in the spawn are difficult to detect before fruiting. Therefore, methods for identifying L. edodes strains and species were developed. Esterase and total protein profiles of the vegetative hyphae were used to identify the strains and also to detect any possible changes in them. Either L. edodes strains or different species of wood-rotting fungi were characterized by changing the pH gradient of the gel in isoelectric focusing. (Copyright (c) Valtion teknillinen tutkimuskeskus (VTT) 1993.)