酶联免疫检测试剂盒检测水产品中孔雀石绿的应用研究

摘要

Malachite green （MG） residues in aquatic products were determined by using ELISA kit. The performance of domestic reagent kit was studied and compared with high performance liquid chromatography method. The results showed that：this kit has good linearity while the content of the malachite green was in the range of 0.5μg/kg- 8μg/kg, the minimum detectable limit was 0.16μg/kg. The total recovery rate was 71% 120% measured by malachite green kit,and the batch coefficient of variation was between 5.61%-18.6%. On detecting of actual samples of mandarin fish,this kit has the same result with high-performance liquid chromatography method, while the quantitative has a varied result. The malachite green residue measured by sample kit was 37.75μg / kg,while that measured by high performance liquid chromatography was 46.29μg / kg. The accuracy deviation range was in line with the State Quality Supervision, Inspection and Quarantine Administration of the＂ quality control＂ requirements for residue analysis. To sum up, homemade enzyme-linked immunity detection reagent box is convenient,fast operation ,high sensitivity,and good repeatability. It is suitable for the rapid detection of a large number of samples.%利用酶联免疫试剂盒检测水产品中孔雀石绿残留量，研究了国产试剂盒检测性能，并与高效液相色谱法进行了比较。结果表明：孔雀石绿含量在0．5μg／kg，8μg／kg范围时，试剂盒具有良好线性，最低检出限为0．16μg／kg；试剂盒测得孔雀石绿总量的加标回收率在7l％-120％，批内变异系数介于5．61％-18．6％；检测鳜鱼实际样品时，试剂盒和高效液相色谱法定性判定结果一致，定量结果有所差异，试荆盒测得鳜鱼样品中孔雀石绿残留量为37．75μg／kg，高效液相色谱法测得结果为46．29μg／kg，但准确度偏差范围尚符合国家质量监督检验检疫总局《残留分析质量控制》的要求。综上所述，国产酶联免疫检测试剂盒操作方便快速，灵敏度较高，重现性较好，适用于大量样品的快速检测。