固体发酵裂褶多糖免疫调节及抗肿瘤活性研究

摘要

以麦麸和玉米芯为原料固体发酵生产裂褶多糖（schizophyllan，SPG），采用辐照技术降解处理SPG（irradiated schizophyllan，ISPG），研究辐照前后裂褶多糖分子量、体内免疫调节及抗肿瘤活性变化。结果显示，辐照能有效降低天然SPG分子量，分子量从106～107 Da（ SPG）降低到104～105 Da（ ISPG）。 SPG和ISPG均具有良好免疫调节和抗肿瘤活性， SPG和ISPG中剂量（100 mg/kg体重·d）和低剂量（50 mg/kg体重·d）组均对S180荷瘤小鼠羊红细胞诱导迟发型变态反应（ DTH）和脾细胞介导羊红细胞定量溶血值（ QHS）具有显著增强功效，ISGP中剂量组（100 mg/kg体重·d）能显著增强小鼠巨噬细胞吞噬鸡红细胞效果，提升小鼠非特异性免疫功能；SPG和ISPG不同剂量组抑瘤率均在45％以上，ISPG中剂量组（100 mg/kg体重·d）抑瘤率最高（74．80％±6．83％）。总体看来，辐照处理后的ISPG比天然SPG具有更好的免疫调节及抗肿瘤活性。%Schizophyllan ( SPG) was produced by the fungus Schizophyllum commune under solid-state fermentation conditions in this study. Irradiation technology was used to treat SPG and molecular weight and biological effects of SPG and irradiated schizophyllan ( ISPG) on the immunity-modulatory and antitumor activities in S180 tumor mice models in vivo were investigated. Irradiation technology decreased significantly the molecular weight of SPG ( P< 0.05) , and the range of molecular weight of SPG and ISPG were 106~107 Da and 104~105 Da, respectively. Both SPG and ISPG with medium dose ( 100 mg/kg) and low dose ( 50 mg/kg) had potent immune-modulatory effects in S180 tumor mice model and exhibited significant effect on delayed-type hypersensitivity (DTH) response and quantitative hemolysis spectrophotometry (QHS) test (P<0.05). Besides, ISPG with low dose (50 mg/kg) elevated significantly the phagocytosis of macrophage (P<0.05). The results still showed that SPG and ISPG had great antitumor effects, and ISPG at medium dose ( 100 mg/kg ) had maximum inhibition rate ( 74. 80%± 6. 83%) . Therefore, ISPG had better immunity-modulatory and antitumor activities than natural SPG.