YB-1基因沉默抑制前列腺癌细胞的增殖和迁移

摘要

Objective To investigate the effect of gene silencing of Y-box binding protein-1(YB-1) by RNA interference on the proliferation and migration in prostate cancer cells lines PC-3 cells .Methods YB-1 siRNAs(pGenesil-1-YB-1-1 and pGenesil-1-YB-1-2) were synthesized and transfected into cloned into the the PC-3 cells by liposome .The expressions of YB-1 were measure by RT-PCR and Western blotting .The proliferation and migration were respectively detected by MTT and Transwell method . Results ThemRNA and protein expressions of YB-1 were significantly decreased by pGenesil-1-YB-1-1 and pGenesil-1-YB-1-2 (P<0 .05) ,compared with the control group ,the inhibition ratio of mRNA expression was 36 .23% and 39 .42% respectively and the inhibition ratio of protein expression was 41 .56% and 55 .33% respectively .The proliferation and migration were significantly decreased by pGenesil-1-YB-1-1 and pGenesil-1-YB-1-2(P<0 .05) .Conclusion YB-1 gene silencing by RNA interference inhibits the proliferation and migration in prostate cancer cells lines PC-3 cells .%目的：探讨RNA干扰沉默Y-盒结合蛋白1（YB-1）对前列腺癌细胞株PC-3细胞增殖和侵袭力的影响。方法将YB-1 siRNA（pGenesil-1-YB-1-1、pGenesil-1-YB-1-2质粒）用脂质体转染PC-3细胞（转染组），以逆转录-PCR（RT-PCR）和蛋白质印迹法（Wesretn blotting）分别检测YB-1 mRNA和蛋白的表达，3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐（MTT）比色法和Transwell小孔实验检测PC-3细胞的增殖和侵袭力。结果与质粒对照组相比，转染组YB-1 mRNA 和蛋白的表达水平明显降低（P＜0．05）。重组质粒pGenesil-1-YB-1-1和pGenesil-1-YB-1-2组mRNA的抑制率分别达36．23％和39．42％，YB-1蛋白的抑制率分别达41．56％和55．33％。与正常对照组相比，转染组细胞增殖明显抑制（ P＜0．05），转染处理的穿膜细胞数明显减少（P＜0．05）。结论 RNA干扰沉默YB-1基因表达降低了前列腺癌细胞的增殖和迁移能力。