麦冬皂苷B对A549细胞株体外黏附、侵袭及迁移的抑制作用及机制研究

摘要

Aim To explore the inhibitory effects of ophiopogonin-B (OP-B ) on cell adhesion,invasion and migration in non-small cell lung cancer (NSCLC) A549 cells in vitro and its possible mechanism.Meth-ods Cell adhesion assay and transwell chamber assay were used to detect the ability of cell adhesion,migra-tion and invasion.qRT-PCR was used to detect the mRNA levels of MMP-2 and 9 .Meanwhile ,Western blot assay was performed to determine the protein levels of MMP-2/9 and p-Akt.Results OP-B significantly inhibited the ability of cell adhesion,invasion and mi-gration in A549 cells at the concentration of 10 μmol· L-1 (P<0.01 ).Meanwhile,it inhibited the mRNA and protein levels of MMP-2 and MMP-9 and down-regulated the phosphorylation of Akt (P <0.0 1 ). Conclusion OP-B inhibits cell adhesion,invasion and migration in A549 cells through down-regulation of the mRNA and protein expression of MMP-2/9 ,and the inhibitory effect on the expression of p-Akt.%目的：探究麦冬皂苷B （ophiopogonin-B，OP-B）抑制非小细胞肺癌细胞株A549黏附、侵袭、迁移的作用及其机制。方法细胞黏附实验、Transwell小室实验分别检测细胞黏附、迁移和侵袭能力。通过荧光实时定量反转录 PCR （qRT-PCR）检测OP-B对A549细胞中MMP-2／9 mRNA表达水平的调控，Western blot法检测细胞中MMP-2／9蛋白及其上游调控蛋白Akt及其磷酸化水平的变化。结果10μmol ·L－1浓度的 OP-B 能明显抑制 A549细胞黏附、侵袭和迁移，同时能抑制其MMP-2／9 mRNA及蛋白水平的表达，并抑制其上游p-Akt的表达。结论 OP-B有效抑制A549细胞黏附、侵袭和迁移，其作用与下调MMP-2／9 mRNA和蛋白水平的表达及抑制其上游Akt的磷酸化有关。