An in vitro experimental model for research of the mechanism of tumor perineural invasion, comprising: a first culture medium supply bottle, a first pump, a first culture chamber, a first waste liquid collection bottle, a second culture medium supply bottle, a second pump, a second culture chamber, and a second waste liquid collection bottle. The first culture medium supply bottle, the first pump, the first culture chamber, and the first waste liquid collection bottle are successively connected; the second culture medium supply bottle, the second pump, the second culture chamber, and the second waste liquid collection bottle are successively connected; a first valve is disposed between the first culture chamber and the second culture chamber; the first culture chamber is located on the left side of the second culture chamber.
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