FSP1在小鼠巨噬细胞中的表达特征及其生物学意义

摘要

Objective To determine the expression of fibroblast-specific protein 1 (FSP1) on macrophages and its roles on macrophage functions. Methods FSP1-GFP reporter mice was used in the present study. Macrophages from FSP1-GFP mice were treated with H2O2 and the FSP1 expression was detected by flow cytometry and ELISA in protein level. The expressions of cytokines and the phagocytosis on FSP1- or FSP1+ macrophages were assayed by flow cytometry. Results Peritoneal macrophages and bone marrow-derived macrophages can be divided into either FSP1+ or FSP1- subpopulations. The percentage of FSP1+ macrophages was decreased and FSP1 in the supernatant was increased after oxidative stress induction by H2O2 stimulation. There was no significant difference between FSP1- and FSP1+ macrophages on the expressions of pro-inflammatory cytokines like IL-6, IL-12 and TNF-α after stimulated with LPS. The phagocytosis on chicken red blood cells and latex beads of FSP1-macrophages was higher than FSP1+ macrophages. ConclusionsMacrophages can be divided into two subpopulations depending on the expression of FSP1. FSP1+macrophages can secret abundant FSP1 protein after H2O2 stimulation. There is no significant difference between the two subpopulations of macrophages on the secret of cytokines, but FSP1- macrophages own higher phagocytosis ability than FSP1+ macrophages.%目的：探讨 FSP1在小鼠巨噬细胞中的表达特征及其在巨噬细胞功能的意义。方法以 FSP1-GFP小鼠作为研究对象，采用流式细胞染色检测 FSP1在小鼠巨噬细胞中的表达情况；应用多种细胞因子对 FSP1-巨噬细胞进行刺激，观察 FSP1的表达情况；应用过氧化氢（H2O2）对巨噬细胞进行氧化应激诱导，并观察其 FSP1的表达情况及上清中 FSP1的浓度；流式检测 FSP1-和 FSP1+的巨噬细胞细胞因子分泌情况及吞噬功能。结果腹腔巨噬细胞和骨髓诱导来源的巨噬细胞均可被分为 FSP1+和 FSP1-两群。随着 H2O2刺激腹腔巨噬细胞时间延长，FSP1+巨噬细胞的比例下降，ELISA 检测上清中的 FSP1浓度增加；刺激 FSP1+和 FSP1-两群巨噬细胞对 LPS诱导的 IL-6、IL-12、TNF-α等促炎性细胞因子无明显差异；FSP1-的腹腔巨噬细胞对鸡红细胞及乳胶微球的吞噬能力高于 FSP1+巨噬细胞。结论依据巨噬细胞对 FSP1的表达可将巨噬细胞分为 FSP1+和 FSP1-两群。FSP1+巨噬细胞经 H2O2刺激分泌大量 FSP1蛋白。这两群巨噬细胞对促炎性细胞因子的分泌无明显差异，但 FSP1-巨噬细胞的吞噬能力高于 FSP1+巨噬细胞。