隐孢子虫感染小鼠MHC-Ⅱ类抗原的表达

摘要

To observe the treating function of Fructus Psoraleae and dihydroartemisinin on mouse infected with Cryptosporidiurn parvum and to research the regulating function of major histocompatibility complex- Ⅱ , 60 mice were randomly divided into five groups: normal group A; positive control group- Ⅰ B;one-week treatment group C; positive control group- Ⅱ D; two-week treatment group E. Mice in group B, C, D, and E were injected subcutaneously with dexamethasone. When the mice were infected with C. parvum, they were treated with the medicine respectively. The intestine tissue was observed via HE.The expression of MHC-Ⅱ were observed by immunohistochemistry. The damaged intestine got improved and repaired, and a significant decrease of the amount of C. parvum oocysts in feces was shown in the treated groups (C, E) compared with positive control groups (B, D) (P＜0. 05). In 1-week treatment group, the MHC- Ⅱ expression of group B was higher than that of normal group A. There was no difference between the expression of MHC- Ⅱ in group B and that in group C; in 2-week treatment group, there was no difference between the expression of MHC- Ⅱ in group E and that in group A. The MHC- Ⅱ expression of group D was lower than that of group A and B (P＜0.05). It's suggested that fructus Psoraleae and dihydroartemisinin are effective to treat mouse cryptosporidiosis. The expression of MHC- Ⅱ might be regulated by the mixture.%目的 探讨中药补骨脂、双氢青蒿素二者配伍对小鼠隐孢子虫病的疗效.研究该合剂对MHC-Ⅱ类抗原表达的调节作用.方法 将60只昆明小鼠随机分成5组:A:正常组;B:阳性对照组1;C:1w治疗组;D:阳性对照组2;E:2w治疗组.B、C、D、E四组小鼠经腹股沟皮下注射地塞米松磷酸钠注射液,建立隐孢子虫感染模型.经1w和2w药物治疗后,计数各组小鼠粪便卵囊数量并观察回肠组织中MHC-Ⅱ类抗原的表达.结果 所有药物治疗组,粪便卵囊量与阳性对照组相比均明显减少(P(0.05).在经药物治疗1w组,B组MHC-Ⅱ类抗原的表达显著高于A组(P(0.05),C组与B组无显著性差异.治疗2w后,E组MHC-Ⅱ类抗原的表达与正常对照组A组无差别(P＞0.05),D组MHC-Ⅱ类抗原的表达显著低于A组(P<0.05).结论 MHC-Ⅱ类抗原在隐孢子虫感染小鼠中表达升高,随着虫体感染加重,MHC-Ⅱ类抗原表达受抑;补骨脂、双氢青蒿素合剂可通过调节MHC-Ⅱ类抗原的表达,进而调节宿主免疫应答反应的能力,达到抑杀虫体,修复病损的目的.