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寄生虫与医学昆虫学报

寄生虫与医学昆虫学报

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Acta Parasitology et Medica Entomologica Sinica
《寄生虫与医学昆虫学报》是由中国动物学会、中国昆虫学会和军事医学科学院微生物流行病研究所主办的、国内寄生虫学和医学昆虫学最高水平的学术刊物。她力求反映我国本学科研究和防治实践的新观点、新方法和新成就，以促进现代科学技术与本学科的融合，加强国内外专业人员的学术交流，推动我国寄生虫学和医学昆虫学向更高水平发展。本刊为本专业人员提供了一块发表研究成果、探讨不同学术观点、进行学术交流和讨论的科学园地。本刊为季刊，主要包括论文、综述、简报、论坛等栏目，主要读者对象包括我国寄生虫学和医学昆虫学科研、教学、临床医疗、卫生防疫、动植物检疫、畜牧兽医、植物保护和医药工业等专业人员和生物学、医学领域及其他相关学科的专业人员和国内外同行学者。本刊自1992年获准开办以来，已出版12卷共49期，向国内外发行。目前已被收录为中国科技论文统计源期刊，并作为《中国核心期刊数据库》收录对象，同时本刊也被美国的《化学文摘》（CA）、英国的《动物学记录》（ZR）、俄罗斯《文摘杂志》（AJ）以及英国国际农业和生物科技中心5种索引期刊收录。
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  • 发文量：1023
  • 被引量：155
  • H指数：1
  • 开始收录时间：1994,1(1)
  • CNKI综合因子：0.566
  • 维普期刊影响因子： 0.65
  • 万方期刊影响因子：0.32
  • 创刊时间：1994
  • 国内刊号/CN：11-3158/R
    国际刊号/ISSN：1005-0507
  • 发行周期：季刊
    邮发代号：80-105
  • 主管单位：军事医学科学院
    主办单位：中国动物学会、中国昆虫学会、军事医学科学院微生物流行病研究所
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  • 邮箱：jscyxkcl@126.com；jscyxkc@126.com
  • 地址：北京丰台东大街20号
  • 邮编：100071
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  • 摘要：肺孢子菌肺炎(PCP)是一种由耶氏肺孢子菌引起的致死性肺炎,常见于免疫功能低下人群,早期诊断有助于临床治疗.目前常用的病原学检查漏诊率较高;定性PCR检测虽然敏感,但有时难以区分隐性感染和显性感染.实时定量PCR是近年来发展的一种精确、敏感、污染少的核酸定量技术.本文综述结果表明,实时定量PCR技术能快速、敏感、特异地检测肺孢子菌,可随时跟踪监测PCP的治疗效果,指导临床用药,有助于PCP的流行病学研究及其他基础生物学研究.%Pneumocystis pneumonia ( PCP) caused by Pneurnocytsis jiroveci ( P. j) is a life-threatening infection in immunocompromised individuals. Early diagnosis of PCP is important to clinical treatment. The diagnostic technique for PCP used nowadays relies on a direct microscopic examination of respiratory specimen, the sensitivities of such examination is low. Molecular diagnosis of PCP is potentially more sensitive. Although conventional PCR has been used to detect the P. j DNA, it is sometimes difficult to distinguish the apparent and silent infection. The real-time PCR assay was developed recent years, which seemed to be accurate, sensitive and less contamination. The present paper summarized more than 20 papers conceming the studies on real-time PCR for the identification of P. j DNA. It revealed that real-time PCR is rapid,sensitive, specific diagnosis method for the detection of P. j. The quantitative results would be helpful to therapy monitoring, guide of clinical drug application and epidemiological investigation for PCP. It is also useful for other basic biological researches.
  • 摘要：黑龙江立克次体为远东蜱传斑点热病原体,本文以黑龙江立克次体基因组为模板,扩增ompB的4段基因,利用原核表达载体构建重组表达质粒,IPTG诱导重组质粒转化的大肠杆菌表达重组目的蛋白,并对其抗原性进行分析.免疫印迹反应表明,重组表达的4个蛋白均与黑龙江立克次体感染小鼠血清反应.该4个重组蛋白分别免疫小鼠后,分析其血清的特异性结果显示,4个重组蛋白免疫血清,其特异性抗体效价均≥1:5 120,与斑点热群立氏立克次体有相对明显的血清学交叉反应(≥1:40),而和斑疹伤寒群立克次体仅有轻度交叉反应(≤1:40).结果表明,重组OmpB蛋白能有效诱导机体产生特异性体液免疫应答,并能与黑龙江立克次体抗体产生特异反应,显示其具有良好的抗原性.%Rickettsia heilongjiangensis is a human pathogen that causea Far East tick-bome spotted fever. Four fragments of outer membrane protein B gene ( ompB) were amplified from R. heilongjiangensis , and the gene fragments were recombined with prokaryotic expression plasmid. Four recombinant OmpB proteins were expressed in E. coli cells transformed with the recombinant plasmids, respectively, following IPTG stimulation. In immunoblot assay, the recombinant OmpB proteins reacted with sera from mice infected with R. heilongjiangensis. In immunofluorescence assay, the sera from mice immunized with each recombinant OmpB protein strongly reacted with R. heilogjiangensis and the specific antibody titers were ≥1: 5 120, and the prominent cross-reactivity was observed between the immune sera and R. rickettsii ( ≥ 1: 40) while the little cross-reactivity was observed between the immune sera and typhus group rickettsiae ( ≤ 1 : 40 ) . The resuhs suggest that the recombinant OmpB proteins have a good antigenicity since they have capahility to efficiently elicit humoral immunoresponses and to specifically react with rickettsial antibodies.
  • 摘要：Viral hemorrhagic fever is a group of natural focus infection diseases caused by different viruaes featured of pyrexia, haemorrhagia, shock and high case-fatality. Some of the diseases are related to arbovirus, including Rift valley fever, Crimean-Congo hemorrhagic fever, Hemorrhagic fever with renal syndrome, Yellow fever, Dengue fever, Kyasanur forest disease, Omsk hemorrhagic fever, and Chikungunya fever. The present paper made a review of the diseases listed above on their epidemic characteristic, etiology, diagnosis, treatment and prevention.%病毒性出血热是由不同病毒引起的以发热、出血、休克、高病死率为特征的一组自然疫源性疾病,其中与虫媒病毒有关的出血热主要有立夫特山谷热、克里米亚刚果出血热、肾综合征出血热、黄热病、登革热、科萨努尔森林病、鄂木斯克出血热、基孔肯雅热等,本文从流行特征、病原学、诊断、治疗及预防等方面对上述疾病作一综述.
  • 摘要：动态观察小鼠人工感染广州管圆线虫并接受阿苯达唑治疗后外周血嗜酸性粒细胞数量、循环抗原CAg、IL-5水平变化,探讨阿苯达唑治疗广州管圆线虫感染的免疫学效应.将160只BALB/c小鼠随机分组成4组:实验组、感染未给药对照Ⅰ组、未感染给药对照Ⅱ组、健康对照Ⅲ组.动态计数各组小鼠外周血嗜酸性粒细胞,采用单抗金标层析法和酶联免疫吸附法测定循环抗原CAg和血清IL-5,并用RT-PCR方法验证脾细胞IL-5 mRNA表达水平的变化.结果发现,实验组和对照Ⅰ组小鼠相比于对照Ⅱ组和对照Ⅲ组的各项指标变化有所差异,实验组的外周血嗜酸性粒细胞增加在第4周达到高峰后随即下降,至第7周已与对照Ⅱ、Ⅲ组无显著性差异.对照Ⅰ组则在第2周开始出现的嗜酸性粒细胞增加一直持续至整个实验周期.实验组的广州管圆线虫CAg阳性率和血清IL-5水平的高峰期和恢复期也均比对照Ⅰ组提前,同时IL-5 mRNA表达水平也呈现相应的变化.本研究结果提示阿苯达唑可改变感染广州管圆线虫后小鼠的外周血嗜酸性粒细胞、循环抗原、IL-5血清浓度等免疫学效应的变化趋势.%Our objective was to dynamically observe the changes of eosinophil counts, circulating antigens, interleukin 5 ( IL-5 ) level and mRNA expression in peripheral blood, so as to explore the immunological effect of abendazuo on treatment of mouse with Angiostrongylus cantonensis infection. 160 BALB/c mice were randomly divided into four groups ( 40 each ).Animals in experimental group were artificially infected with Angiostrongylus cantonensis and treated with abendazuo, in control I group were also artificially infected without treatment, in control Ⅱ group were not infected but admitted with abendazuo, and in control Ⅲ group were neither infected nor treated. The results showed that the number of eosinophil increased in hoth experimental and control Ⅰ groups. The eosinophil count was dramatically higher in experimental group than in control Ⅰ group at the 4th week, and then decreased to normal level at the 7th week. Meanwhile , the emergence of the peak values of CAg and serum IL-5 level was advanced in the experimental group than in the control Ⅰ group. RT-PCR results for the expression of IL-5 mRNA also showed the same change. The change tendency of eosinophil number,circulating antigens, IL-5 level and mRNA expression may be influenced by abendazuo administration.
  • 摘要：为了解新疆伊犁那拉提草原啮齿动物中自然感染恙虫病东方体(Ot)的情况,在那拉提草原捕获鼠和旱獭,分别取其脾组织提取DNA,应用巢式PCR(nPCR)方法检测Ot-Sta56基因;基因分型引物扩增阳性片段,判断该地区存在的Ot基因型,部分阳性标本测序,通过NCBI网站对序列进行BLAST比较分析.结果显示,在该地区共捕获鼠67只,旱獭29只,在其中3只鼠、2只旱獭的脾中检测到阳性片段,基因型包括Karp型、Saitama型,BLAST结果显示其中1个片段的核苷酸序列与Karp型同源性为97%,另1个片段未能测出核苷酸序列.推测在那拉提地区鼠类可能感染Ot,基因型为Karp型,怀疑可能有Saitama型存在.%DNAs were extracted from spleens of the rodents captured from Nalati area of Xinjiang Uygur Auto. Reg. , and nested-polymerase chain reaction ( nPCR ) was performed to detect the Orientia tsutsugamushi ( Ot )-Sta 56 gene. Some positive samples were sequenced and analyzed by BLAST of NCBI network. A total of 67 rodents and 29 marmots were captured in Nalati area. The positive fragments were detected from the spleens of 3 mice and 2 marmots , including Karp and Saitama genotype. The detection rates of Ot from Mus musculus, Cricetulus migratorius and Marmota barbacina were 6. 06％, 2. 94％ and 6. 90％ , respectively. The homology of one fragment of the amplified gene nucleotide sequence to Karp strain was 99％ . The other fragments could not be sequenced. It was presumed that the Karp strain was the main gene type, and the Saitama strain might exist in this area.
  • 摘要：Coxiella burnetii is the etiological agent of Q fever. Nineteen genes encoding sero-reactive proteins amplified by PCR were inserted into vectors and expressed in E. coli. The recombinant proteins were purified by Ni-NTA affinity chromatography and used to prepare protein microarray. The protein microarray was probed with sera from mice experimentally infected with C. burnetii, and 0, 16, 19 and 18 recombinant proteins on the microarray were recognized by mouse sera collected on week 1, 2, 3 and 4 post-infection, respectively. The results indicated that the proteins on the microarray are specifically reactive to antibodies against C. burnetii, and may be used for Q fever sero-diagnosis.%贝氏柯克斯体为Q热的病原菌.本研究对19个贝氏柯克斯体血清学反应阳性蛋白基因进行PCR扩增,将扩得的基因片段与表达载体连接,将重组质粒转化大肠杆菌并表达目的蛋白,采用Ni-NTA亲和层析柱纯化目的蛋白.将纯化的重组蛋白点在醛基化玻片上制备蛋白芯片.将贝氏柯克斯体新桥株实验感染小鼠血清分别对蛋白芯片进行血清学分析,结果显示能与贝氏柯克斯体感染1、2、3、4周小鼠血清反应的蛋白数分别是0、16、19和18个.结果说明该芯片上的重组蛋白具有贝氏柯克斯体感染血清反应特异性,该芯片分析有望用于Q热的血清学诊断.
  • 摘要：From 1 day-old to 25 day-old adults of house fly , Musca domestica ( Diptera : Muscidae ), 7 groups were chosen and at the temperature of 25℃ ( RH 45％ ～ 55％ ), the flight capacity ( FC ) were measured in flight mills. In view of Accumulative Flight Distance ( AFDi ), both male and female shows 2 peaks of flight capacity on 9 day-old and 17 day-old.In view of Accumulative Flight Duration ( AFDu ), the male represents 1 peak of flight capacity on 17 day-old, and the female represents 2 peaks of flight capacity on 5 day-old and 17 day-old. In view of Average of Flight Velocity ( AFV ), both sexes show a valley on 5 day-old and keep high levels on 9, 13 and 17 day-old. The result shows prior to 5 day-old, male adults are greatly weaker than female in same day-old. From 9 day-old to 17 day-old, FC of house fly adults is in high level except the 13 day-old adults. According to AFDi of each takeoff or AFDu of each takeoff, the lowest of FC occurs at 21 day-old in male, and at 25 day-old in female; FC at 1 dav-old is remarkably lower than that at 5 day-old both in female andmale; in male the summit of FC occurs at 17 day-old, and the sub-summit of FC occurs at 9 day-old. According to AFV,both in male and female, FC at 5 day-old is lowest; FC with summit occurs at 9 day-old in male, but 2 summits of FC occur at 9 day-old and 17 day-old in female. Only according to AFDi, summit of FC in female occurs at 9 day-old, and subsummit occurs on 17 day-old. The result shows that in both sexes the AFDi of 17 day-old adult is different with that of both day-old and 21 day-old in extreme siginicance ( F( 0.01 ) ＝ 3. 05 ＞ F( 6. 102 ＝ 2. 99 ). The result also shows that the AFV of males is different with that of females significantly ( F( 0.05 ) ＝ 4. 61 ＞ F( 1. 108 ) ＝ 3. 93 ).%25℃条件下(RH 45%～55%),对家蝇驯化种群1～25日龄的7个日龄组的雌、雄成虫进行吊飞研究,从累计飞行距离看,雌、雄成虫均为双峰型,在9日龄和17日龄,飞行机能分别达到2个高峰.从累计飞行时间看,雄虫为单峰型,在17日龄达到高峰,雌虫为双峰型,在5日龄和17日龄飞行机能达到高峰.从平均飞行速度来看,雌、雄成虫均在5日龄出现1个最低值,在9、13、17日龄,飞行机能处于高水平.综合来看,发现5日龄以前雄虫的飞行能力远不如雌虫;9~17日龄家蝇飞行机能维持在较高水平;17日龄后飞行机能显著衰退.从累计飞行距离和累计飞行时间来看,雄虫飞行机能的最弱期发生在21日龄,雌虫发生在25日龄;1日龄的飞行机能不论雌、雄均不如5日龄;雄虫的飞行机能高峰值发生在17日龄,9日龄为第2峰值.从平均飞行速度来看,不论雌、雄,家蝇成虫飞行机能在5日龄均为最弱,甚至弱于21日龄;雄虫的飞行机能最高峰出现在第9日龄,雌虫的最高峰同时出现在第9日龄和第17日龄.单从累计飞行距离来看,雌虫的飞行机能高峰值发生在9日龄,17日龄为第2峰值.统计分析结果显示,家蝇累计吊飞飞行距离在17日龄组与1日龄组和21日龄组间均有极其显著的差异(F(0.01)=3.05>F(6,102)=2.99);吊飞飞行速度在雌、雄不同性别间有显著的差异(F(0.05)=4.61>F(1,108)=3.93).
  • 摘要：随着地理信息系统(GIS)理论的产生发展以及方法和技术的成熟,其在血吸虫病防治和研究中的应用也逐步深入.本文就GIS在血吸虫病领域的应用进行综述.%With the perfectibility of high technology and development of software , geographic information system ( GIS ) has being applied and developed in the epidemiology and control of schistosomiasis. The research progress and application of GIS in the field of schistosomiasis control is herewith reviewed in present paper.
  • 摘要：本文记述了采自我国陕西省凤县雄性宝鸡虻Tabanus baojiensis Xu et Liu.标本保存于兰州军区疾病预防控制中心昆虫标本室.%The present paper deals with the male Tabanus baojiensis Xu et Liu from Feng County, Shaanxi Province , China.The specimens are preserved in the Medical Entomology Collection Gallery of Center for Disease Control and Prevention of Lanzhou Command, Lanzhou, China. The specimen of male T. baojiensis is somewhat similar to Tabanus yao Macq. , 1855 ,but differ from the latter chiefly in the color and hairs of the palpus .
  • 摘要：对采自安徽、江苏、湖南、广西、云南及海南等地区的人房、牛棚、稻田、山区等不同生境的按蚊,按照文献合成引物,用PCR方法进行分子鉴定;并对各种群的ITS2序列测序后,与GenBank所得赫坎按蚊复合体近缘种群按蚊的ITS2序列进行比对分析.结果显示,采集的不同地区不同生境的按蚊均为中华按蚊,此次采集没有采到嗜人按蚊;现场的中华按蚊与凉山按蚊、贵阳按蚊和昆明按蚊的同源性为80.6%~83.1%,与嗜人按蚊和雷氏按蚊的同源性为75.2%~76.2%,与最黑按蚊和带足按蚊的同源性稍低,为63.2%~64.6%.系统发育树显示:所有的序列样本形成了两个分支,来自现场的按蚊处在同一分支且差异小.%The partial rDNA-ITS2 segments of Anopheles from Jiangsu, Anhui, Hunan, Yunnan, Hainan provinces and Guangxi Zhuang autonomous region were amplified using PCR and then were sequenced. The sequence alignment results of rDNA-ITS2 indicated that all the samples obtained from different provinces and habitats belonged to Anopheles sinensis.There were high identities between the samples group which ranging from 98. 2％ to 100％ . The identities between the samples and An. hycauns ( An. liangshanensis, An. kunmingensis , An. lesteri and An. anrhropophagus , An. nigerrimus An.peditaeniatus ) were 79. 1％ -82％ , 76. 1％ -77. 1％ , 60. 9％ -63. 9％ , respectively. Phylogenetic analysis results showed that all the segments obtained from the fields were clustered together and formed two separated branches with the segments of An. hycauns form GenBank.
  • 摘要：This paper analyzed the resistance to Bacillus thuringensis ( BTH-14 ) of Culex pipiens pallens larvae from different growing environment in Jining. The method of liquid immerse was adopted. The LC5o value to BTH-14 of mosquito larvae from different growing environment were mensurated in laboratory. BTH-14 had different killing effect on Cx. pipiens pallens larvae collecting from five habitat. The lethal concentration of mosquito larvae ( A ～ E ) were 17 , 11 , 13 , 7 and 35 mg/L. It was shown that the mosquito larvae from the hospital gutter were the most sensitive to BTH-14 followed by mosquito larvae form the hennery gutter. It was worth mentioning that the larvae collected form the reed pit exhibited the resistance to BTH-14. But the generation of them could not inherit the resistance. The growing environment may effect the sensitivity of mosquito larvae. The resistance of Cx. pipiens pallens larva to BTH-14 could not be passed down.%通过苏云金杆菌对采集的几株野外淡色库蚊种群幼虫的灭杀作用,分析不同环境下,该蚊对苏云金杆菌抗药性的差异.采用幼虫液浸法,测定不同淡色库蚊种群幼虫对苏云金杆菌的LC50.结果显示,苏云金杆菌BTH-14对采集的几株淡色库蚊种群幼虫有不同的灭杀效果,其中采集于苇坑种群的试虫对苏云金杆菌表现出一定程度的抗性,但到F2代,该种群抗性消失,表明这种抗性不能通过遗传形成持续抗性.结果表明不同的孳生环境会影响淡色库蚊幼虫对苏云金杆菌的抗药性,但其抗性不能遗传给后代.
  • 摘要：2011年4月22～24日，由首都医科大学附属北京友谊医院、北京热带医学研究所主办，《中国病原生物学杂志》、《寄生虫与医学昆虫学报》、《中国热带医学杂志》、《中国人兽共患病学报》、《国际医学寄生虫病杂志》协办的国家级继续医学教育项目——第三届北京热带医学与寄生虫学论坛在北京召开，该领域著名专家、学者共60余人参会。论坛开幕式由北京热带医学研究所吕盈盈副研究员主持，所长助理谷俊朝教授和国际著名麻风病专家李桓英研究员分别致辞。
  • 摘要：To investigate the effect of radical treatment in early phase of primary infection with Plasmodium spp. on humoral immune response to homologous reinfection, DBA/2 mice were infected by intraperitoneal injection of 1 x 106 P. berghei ANKA parasited erythrocytes, and radically treated with chloroquine plus artesunate on day 3 after infection. The mice were reinfected with P. berghei ANKA on day 90 after primary infection. The level of parasitemia during primary and secondary infection was observed by means of the Giemsa staining of thin hlood smears, and applying flow cytometry to quantitively analyze the percentage of activated B cells in spleen cell population at day 0, 1 , 3 and 5 post-reinfection, respectively , the levels of specific IgG and its subclasses IgG1 , IgG2a as well as IgE Abs in sera were also measured by ELISA at the same time. It was found that, after reinfection, parasitemia in radically treated mice was transient and extremely low, and that percentage of activated B cells in these mice began increase on day 3 post-reinfection, also, their levels of specific IgG and its subclasses lgG1 , IgG2a simultaneously began to increase, moreover, the levels of IgG, IgC1 and IgG2a from radically treated mice were higher than that in normal mice ( non-infected ) before reinfection, and IgG2a were always higher than IgG1 at each time point, IgE showed a significant increase on day 1 post-reinfection. These results suggested that radical treatment in early phase of primary infection with Plasmodium spp. took less effect on humoral immune response to homologous reinfection. The persistence of some memory B cells and long-lived plasma cells might be important for the host against homologous reinfection, specific IgG, particularly its subclasses IgG2a, and might be the key factor involved in protective immunity against homologous reinfection.%为探讨疟疾感染早期根治性治疗对再感染体液免疫应答的影响,用伯氏疟原虫感染DBA/2小鼠,感染后3天进行根治性治疗,并于初次感染后90天再进行感染.通过薄血膜吉姆萨染色法计数红细胞感染率,流式细胞术检测再感染前(0天)和再感染后(1、3、5天)不同时间点脾细胞中活化性B细胞百分率,ELISA检测血清中特异性IgG、IgG1、IgG2a和总IgE水平.结果发现,同源疟原虫再感染后,根治性治疗小鼠仅出现短暂的低水平原虫血症,再感染后第3天活化性B细胞开始明显升高;同时IgG、IgG1和IgG2a也出现有意义的升高,但再感染前,这些小鼠血清IgG、IgG1和IgG2a均明显高于未感染的正常小鼠,IgG2a在每一检测点均显著高于IgG1,血清中总IgE水平于再感染后第1天即出现明显升高.此结果提示,疟疾感染早期的根治性治疗并不影响宿主在再感染时产生有效的体液免疫应答,一些记忆性B细胞和长效浆细胞的存在可能是抵御再感染的关键因素,特异性IgG,尤其是IgG2a的作用或许尤为重要.
  • 摘要：为了研究表没食子儿茶素没食子酸酯(Epigallocatechin-3-gallate,EGCG)在活体内对疟原虫的抑制,实验选取了感染虎纹伯劳Lanius tigrinus的伯劳疟原虫Plasmodium (Novyella) lanius作为研究对象.实验把通过血转种成功的50只带虫金丝鸟Serinus canaria分成5个组,每组10只进行实验.每组以不同剂量的EGCG进行灌喂实验,设蒿甲醚和生理盐水的阳性和空白对照,并用常规涂片法和PCR法进行抑制效果的检测.实验结果显示在不同剂量下,EGCG在体内对疟原虫生长的抑制率分别是66.7%、90%、90%.不同实验组与空白对照组有显著差异(P <0.001),证实儿茶素在体内对鸟类疟原虫有抑制作用.%To explore the inhibitory effect of epigallocatechin-3-gallate ( EGCG ) on malarial parasite growth in vivo. Fifty canaries ( Serinus canaria ) , which were successfully inoculated with blood sample of a natural host, Lanius tigrinus infected by Plasmodium lanius, were assigned into 5 groups ( 10 each ). Canaries in group Ⅰ , Ⅱ and Ⅲ were orally admitted EGCG 30. 0, 60. 0 and 120. 0 mg/ ( kg · d ), respectively; in group Ⅳ and Ⅴ were orally admitted artemether 50. 0 mg/ ( kg · d ) or normal saline, separately. Results of Giemsa's staining and PCR examination showed the Inhibitive rates of EGCG in group Ⅰ , Ⅱ and Ⅲ were 66. 7％ , 90％ and 90％ , respectively; in group Ⅳ and Ⅴ were 80％ and 0,separately. Very significant differences existed hetween the tested groups ( group Ⅰ , Ⅱ , Ⅲ and Ⅳ ) and the control group ( group Ⅴ , P ＜ 0. 001 ), suggesting that EGCG played an inhibitive effect to Plasmodium ( Novyella ) lanius in Serinus canaria .
  • 摘要：为了探讨不同剂量吡喹酮对脑囊虫病的疗效,将确诊为脑囊虫病者分为2组,第1组吡喹酮剂量为每疗程总量310 mg/kg,分7 d、每日3次口服,共5个疗程,每疗程间歇3个月;第2组吡喹酮剂量为每疗程总量180 mg·kg-1,疗程相同.以临床症状、影像学及免疫学指标作为疗效评价.结果显示,第1组治愈40例,治愈率47.1%,显效43例,显效率50.6%,有效率97.6%;第2组治愈9例,治愈率25.7%,显效20例,显效率57.1%,有效率82.8%,两组的治愈率和有效率均达显著水平(P<0.05).说明应用口服大剂量吡喹酮、多疗程、间歇治疗脑囊虫治愈率高、效果稳定.%To investigate the efficacy of different praziquantel in treating cerehral cysticecosis, the patients with cerebral cysticercosis was divided into two groups. Total dose of praziquantel in each period of treatment in group one was given 310 mg/kg during 7 days, three times a day, for 5 courses and 3 months interval period. Total dose of praziquantel in group two was given 180 mg/kg, same program as group one. The evaluation of curative effect was on the basis of index of clinical symptom, iconography and immunology. The results showed that cure rate , obvious effective rate and total effective rate were 47. 1％ , 50. 6 and 97. 6％ in group one , and 25. 7％ , 57. 1％ and 82. 8％ in group two respectively. Statistical significant differences were observed in the two groups ( P ＜ 0. 05 ). It was illustrated that the cure rate of cerebral cysticercosis by taking high dose of praziquantel orally, and multiple course and intermittent treatment not only was high but also effect was stable.
  • 摘要：近年来国内外科学家将果蝇作为一种模式生物进行睡眠研究,希望能够通过对这种相对简单的生物的睡眠过程的研究,加速人类对睡眠认识的进程.本实验观察四逆散冻干粉对睡眠剥夺模型果蝇的睡眠改善作用.采用光照刺激剥夺睡眠和机械刺激剥夺睡眠两种方法复制果蝇睡眠剥夺模型,利用果蝇DAMS监测器和数据采集自动记录系统评价,以果蝇24 h累积睡眠时间为观察指标,观察各组果蝇睡眠时间.结果显示,与空白组比较,睡眠剥夺组果蝇睡眠时间明显减少,具有显著性差异(P<0.05),说明果蝇睡眠剥夺模型造模成功;与睡眠剥夺模型组比较,给药组果蝇睡眠时间明显延长,具有极显著性差异(P<0.01),说明四逆散冻干粉对两种睡眠剥夺模型果蝇睡眠均有一定的改善作用.%In recent years, Drosophila is a model organism for sleep by domestic and foreign scientists, to further understand human sleep, through this relatively simple biological processes of sleep. The experimental observation of Lyophilized Powder of Sinisan on sleep deprivation ( SD ) improve Drosophila Sleep Time. SD by light stimulation and mechanical stimulation are two ways to copy models, using DAMS monitors and data acquisition system to evaluate flies 24 h accumulated sleep time, it was observed to result in each group. The results show that compared with the control group,SD group flies sleep time was significantly reduced, with significant difference ( P ＜ 0. 05 ), this result indicates Drosophila SD model was successful, and compared with SD group, treatment group was significantly longer sleep time, with highly significant difference ( P ＜ 0. 01 ), this result indicates Lyophilized Powder of Sinisan on SD improve Drosophila Sleep Time.
  • 摘要：The present paper deals with two new species and a new record of biting midges from Mt. Emei, Emei County,Sichuan Province, China. All specimens were collected with net trap during Sept. 15-29 , 2010 from Mt. Emei ( 29. 5°N,103. 3°E ), Sichuan Province, and are deposited in the Collection of Medical Entomology of Beijing Institution of Microbiology and Epidemiology ( 20 Dongdajie Ave. , Fengtai Dis. , Beijing 100071 , China ).1 Atrichopogon emeiensis Deng, Liao et Yu, sp. nov. ♂ ( Fig. 1 )Compound eyes bare, wing length 2. 07 mm, width 0. 55 mm. Scutellum with 6 long bristles, hind tibia with 9 terminal bristles.This new species is quite specific in very short antenna segment 15 , differing distinctly from that of other species of the genus. The new species is closely allied to Atrichopogon schizonyx Giles et Wirth, 1982 and Atrichopogon setosilateralis Borkent et Picado, 2004 , but eyes are pubescent in the allied species, distinctly differing from the new species.Type: holotype ♂ .2 Stilobezzia lijiangi Yu, Zhang et Mo, sp. nov. ♂♀ ( Fig. 2 )Compound eyes bare, wing unmarked, surface without macrotrichia; length ♂ 1. 09 mm, ♀ 1. 15 mm;width ♂ 0. 32 mm ♀ 0. 45 mm, Scutellum with 4 long bristles, hind tibia with 5 terminal bristles.The male of new species is somewhat related to Stilobezzia blaesopira Yu et Deng, 2005. and St. unifaciatus Tokunaga, 1963 , especially in the aedeagus, but distinctly different from the related species in the wing surface without macrotrichia and mid leg TR ＞3. Types: holotype ♂ , paratype ♀ .3 Stilobezzia robusta Das Gupta et Wirth, 1968 ( Fig. 3 ) New record in China. Material exanmined : 1 ♂ .Distribution : Malaysia ; China.%2010年9月中下旬在四川省峨眉县峨眉山(29.5°N,103.3°E)西南麓进行了挥网采集,获得蠓类2亚科10属11种,其中有2个新种:峨眉裸蠓(Atrichopogon emeiensis Deng,Liao et Yu sp.nov.)和李江柱蠓(Stilobezzia lijiangi Yu,Zhang et Mo,sp.nov.);以及1种我国新纪录:柔软柱蠓(Stilobezzia robusta Das Gupta et Wirth).同时发现趋光蠛蠓(La.phototropia Yu et Liu)雄虫和泸定毛蠓(Dasyhelea ludingensis)的雌虫在黄昏时分参与雄性摇蚊的群舞团.本文描述新种模式标本都收藏于军事医学科学院微生物流行病研究所医学昆虫标本馆.
  • 摘要：Two collection methods, the CO2 lamp-trapping and the men-net-bait, were taken to study the population composition of mosquitoes in Beiwan area, the lower reaches of the Erqis River. Aedes vexans was the predominant species among the 8 species of 4 genera distributed in this area. The effectiveness of the two collection methods showed no significant difference on mosquito species captured and species composition in different habitats, but the number of mosquitoes captured was different, implying that the CO2 lamp-trapping is alternative and reliable on the research of mosquitoes population composition.%分别采用人帐诱法和CO2灯诱法对新疆北湾地区不同生境的蚊虫种类组成及构成进行调查并对两种调查方法进行比较.新疆北湾地区蚊虫种类有4属8种,刺扰伊蚊为各种生境优势蚊种,米赛按蚊、里海伊蚊、背点伊蚊以及凶小库蚊为常见种,环跗曼蚊、赫坎按蚊和尖音库蚊为少见种,不同生境蚊虫密度及种类组成不同;灯诱和人帐诱两种方法比较结果显示,两种方法捕获的蚊虫种类及在不同生境中的分布基本一致,但是不同方法捕获的蚊虫数量不同,种类构成比例也有一定变化.研究表明CO2灯诱法也可作为蚊虫种类组成及构成的调查方法.
  • 摘要：评价敌敌畏和高效氯氰菊酯两种药剂亚致死剂量对德国小蠊种群繁殖力的影响.室内测定了两种药剂亚致死剂量对德国小蠊F0代成虫的干扰作用,通过组建两种杀虫剂亚致死剂量影响下德国小蠊F1代的实验种群生命表系统分析了药剂对德国小蠊繁殖力的影响.结果表明在实验室条件下,敌敌畏和高效氯氰菊酯亚致死剂量处理的德国小蠊每代平均产卵荚数及产卵数分别为2.67、2.33枚和38.00、34.33只,对照组德国小蠊平均产卵荚数和产卵数分别为3.67枚和42.00只;对照组德国小蠊雌虫寿命、产卵前期和产卵间隔期分别为120.30、27.07和24.57天,处理组(敌敌畏、高效氯氰菊酯)德国小蠊雌虫寿命分别为112.30、93.73天,而产卵前期、产卵间隔期分别为31.63、30.37和28.80、31.77天,与对照组具有统计学差异.在实验室条件下两种药剂对德国小蠊F0代是通过缩短成虫寿命、减少雌虫产卵量和延长产卵间隔期来影响F0代种群数量的.而两种药剂亚致死剂量胁迫下对德国小蠊F1代种群动态的后续影响表现为F1代种群的内禀增长率、净增殖率和周限增长率降低,平均世代历期和种群加倍时间延长.研究结果表明两种药剂亚致死剂量对德国小蠊具有持续控制作用,不存在刺激德国小蠊增殖的现象.%To assess the effects of sublethal dosage of dichlorvos and beta-cypermethrin on the population productivity of Blattella germanica. Laboratory tests were conducted to assess the effects of sublethal dosage of the two insecticides on F0 generation of adult B. germanica. By comparing the life table of F1 generation, the effects of the two insecticides on the population dynamics of B. germanica were analyzed. After treatment of sublethal dosage of dichlorvos and beta-cypermethrin , the average ootheca number each female laid and hatched number of each ootheca of F0 generation were 2. 67 and 2. 33, and 38. 00/ootheca and 34. 33/ootheca, respectively, and the controls were 3. 67 and 42. 00/ootheca. The longevity , preoviposition period and oviposition interval period of female adults treated with dichlorvos or beta-cypermethrin were 112. 30d and 93. 73d, 31. 63d and 30. 37d, and 28. 80d and 31. 77d, respectively , statistically different compared to those of controls ( 120. 30d, 27. 07d and 24. 57d, respectively) . For F0 generation, the essential effects of sublethal dosage of the two insecticides were shortening the longevity, depressing the fecundity and extending the interval period of oviposition; For F1 generation, the followed influence were on depressing rm , R0 and λ , as well as extending Td and td . The sublethal dosage of dichlorvos and beta-cypermethrin didn't lead to the increase of B. germanica population, implying that the suhlethal dosage of dichlorvos and beta-cypermethrin may control the B. germanica population continuously.
  • 摘要：A new species of Culicoides, C. jiulongensis Liu et Wang sp. nov. is described from Sichuan Province, China. The new species is closely allied to Culicoides sublatifrontis Smatov et Isimbekov 1971, but is distinctly different from the spermathecae 2 subequal, antennal sensilla coeloconica present on segments 3, 7, (10)11-14, mandible with 16 teeth, and maxilla with 21 teeth, and H/P 1.03 of female C. sublatifrontis Smatov et Isimbekov, 1971. The new species is also similar to Culicoides latifrontis Shakrizjanova, 1962, but is distinctly different from the AR 1.00(0.94-1.10),PR 1.84 (1.33-2.00), mandible with 15-17 teeth of female C. latifrontis shakriajanova, 1962. A new record in China C.abchazicus Dzhafarov 1964 is described from Liaoning, Jiling Province and Inner Mongolia Autonomous Region. All specimens are deposited in the Centre for Disease Control and Prevention of Shenyang Command, Shenyang 110034, China.%报道了采自四川省九龙县库蠓属一新种:九龙库蠓Culicoides jiulongensis Liu et Wang sp.nov.,本新种与Culicoides sublatifrontis Smatov et Isimbekov,1971 相近似,但后者雌虫2个受精囊等大,触角嗅觉器见于第3,7,(10),11～14,大颚齿16枚,小颚齿21枚,头喙比(H/P)1.03与本新种明显不相同;本新种与Culicoides latifrontis Shakrizjanova,1962也相近似,但后者触角比(AR)1.00 (0.94～1.10),触须比(PR)1.84 (1.33～2.00),大颚齿15～17枚与本新种明显不同.新发现了中国新纪录种:切割库蠓Culicoides abchazicus Dzhafarov,1964.模式标本保存在沈阳军区疾病预防控制中心,沈阳 110034.
  • 摘要：本研究的目的是采用荧光定量PCR扩增技术,建立快速检测淡色库蚊Culex pipiens pallens钠离子通道L1014位点击倒抗性(Knockdown resistance,kdr)相关点突变方法,该方法的原理是以突变位点为3′端设计两条特异性上游引物,和一条非特异下游引物组成两对引物平行双管法,用荧光定量PCR扩增快速检测淡色库蚊钠通道L1014F突变的SS,SR,RR 3种基因型.用此方法对48个北京淡色库蚊样本进行基因分型,42个样本检测结果与等位基因特异性PCR(AS-PCR)检测结果完全一致,得到SS(TTA/TTA)基因型21个,占50.0%,SR(TTA/TTT)基因型16个,占38.1%,RR(TTT/TTT)基因型5个,占11.9%,与测序结果完全一致;另外6个样本检测结果与AS-PCR检测结果不一致,测序显示:TCA(L1014S)、TTC(L1014F)突变各1株,另4株为敏感纯合子.因此,PCR SYBR GREEN 扩增灵敏度(93.75%)低于AS-PCR法(100%),但特异性较高,达100%;分析原因可能是被检测样本引物结合部位多态性较高,影响了引物的结合,而AS-PCR凭经验能完成部分结果的判断.综上所述,用PCR SYBR GREEN 扩增技术快速检测淡色库蚊的L1014F基因突变仍是一种值得采纳的等位基因分型方法.%To set up a real-time fluorescent quantitative PCR assay for rapid detection point mutation ( TTA-TTT of L1014F) in Culex pipiens pallens sodium channel gene-related with knockdown resistance ( kdr) . Two paired-primers with 3 '-basyl of sense-primer comhined on the mutation point were contrived in parallel for each specimen. The specimens can be easily genotyped as SS, RS, RR as same as the AS-PCR did when there was no polymorphism in the target sequence complemented with primers. Of 48 specimens were detected in this assay , 42 specimens showed the same results as AS-PCR did gaining 21 SS genotypes (50. 0％ ) , 16 SR genotypes (38. 1％ ) and 5 RR genotypes ( 11. 9％ ) . There were 2 of other 6 specimens showed substitutions from TTA to TGA and TTC in the allele of L1014 , and 4 SS genotypes in other 4 specimens with polymorphism in the target sequence according to sequencing results. So in brief, the PCR assay showed lower sensitivity (93. 75％ ) than AS-PCR did ( 100％ ) , but higher specificity ( 100％ ) according to direct sequencing.
  • 摘要：The sequence of PfEMP1 ( FCR3S1. 2-var1) DBLα were optimized according to the characteristics of E. coli codon and synthesized, and then divided it into three fragments by PCR. Full-length gene and three gene fragments were expressed in E. coli and then purified. In order to identify if recombinant DBLα protein have specific affinity with blood group antigen A/B , the recombinant proteins were incubated with A/B-tri-APE-gel, the protein bound in the A/B-tri-APE-gel hinding protein was washed with PBST buffer before running for 10％ SDS-PAGE. The results showed that recomhination PfEMP1 ( FCR3S1. 2-var1 ) DBLα protein and different fragments can specifically bind blood group antigen A/B, which confirmed the blood group antigen A/B as one of the receptors for Rosetting.%根据大肠杆菌密码子组成特点,重新优化并合成FCR3S1.2株恶性疟原虫红细胞表膜蛋白1(PfEMP1)DBLα区基因序列,利用PCR的方法,将优化后的基因序列分为3段.分别将全长基因和3个基因片段在大肠杆菌中表达、纯化.通过重组蛋白与血型抗原的亲和试验,分析功能区与血型抗原是否具有亲和力.结果表明,重组的PfEMP1-DBLα全长蛋白质及分段表达的重组蛋白对血型抗原A、B均有特异性的亲和力.本实验结果为揭示恶性疟原虫的主要致病分子与人体细胞相互作用机制提供了实验依据.
  • 摘要：P47 is a protein expressed only in female gametocyte and located on the surface of female gametes of malaria parasites. In present study, the complete sequence of Plasmodium yoelii Pys47 was amplified by PCR and cloned into eukaryotic expression vector pcDNA3. 1＋. Cene sequencing and restriction enzyme digestion confirmed the successful construction of Pys47 plasmid. The transmission blocking effect of immune sera from BALB/c mice vaccinated with the DNA plasmid Pys47 was determined by IFA, but the anti-sera couldn't markedly inhibit the development of the protozoa from gametocyte into zygotes and ookinetes when the parasites in sexual stage were cultured with anti-sera in vitro. The results of present study implied that the specific surface protein Pys47 may not be an ideal candidate of immunizing antigen for transmission-blocking vaccine.%P47是特异性表达于疟原虫雌性配子体以及雌性配子表面的蛋白,为了评价其作为传播阻断疫苗候选抗原的免疫效果及其效应,本研究将约氏疟原虫Pys47完整编码区基因序列克隆入真核表达载体pcDNA3.1+,获得核酸质粒,肌肉注射BALB/c小鼠.通过ELISA方法检测免疫小鼠血清滴度,IFA检测疫苗的免疫活性以及免疫血清对有性阶段虫体发育的阻断效应.测序结果和酶切鉴定证实构建的质粒与要求相符;ELISA结果显示免疫小鼠血清特异性抗体滴度明显高于对照组;IFA结果显示免疫血清未能明显阻断配子体向合子与动合子的发育.因此,本实验提示作为疟原虫有性阶段特异性表达蛋白P47,可能不是一个理想的疟原虫传播阻断疫苗候选抗原.
  • 摘要：本文补充描述狭谷绳蚋Simulium (Gomphostilbia) synanceium Chen et Cao,1983,幼虫.%A detailed description of the larva of Simulium (Gomphostilbia) synanceium Chen et Cao, 1983, which did not be described before is given, collected from the Fengcheng City, and Fuxin City, Liaoning Province, China.
  • 摘要：兔隐孢子虫是新近发现的人兽共患隐孢子虫种,已引起全球关注.本文对其分类地位、流行病学及分子特征研究等方面进行综述.%Cryptosporidium cuniculus in rabbits is novel and zoonotic parasite. It has heen recognized all over the world.The aim of this present paper is to summarize the current status of knowledge about Cryptosporidium cuniculus , including the classification position , epidemiology and molecular characterizations.
  • 摘要：The biological characteristics of deltamethrin-resistant and susceptible strains of Aedes albopictus were studied,and SDS-PAGE electrophoresis was employed to investigate the difference of expressed proteins from Ⅳ-instar larvae and adults of the two strains of mosquitoes. The results showed that the pupation rate of resistant strain was higher than that of susceptible strain ( P ＜ 0. 05 ) , but the emergence rate of two strains was not significantly different ( P ＞ 0. 05 ) . It was revealed by SDS-PAGE that the protein bands of adult were significantly different from that of Ⅳ -instar larvae with a specific band with about 200 kDa detected in adult individuals. However, no significant difference was found in the soluble protein pattern of both larvae and adults between the two strains.%在实验室条件下观察白纹伊蚊溴氰菊酯敏感品系与抗性品系的生物学特性,并对两品系的Ⅳ龄幼虫和成蚊的可溶性蛋白提取物进行SDS-PAGE分析.结果表明:与敏感品系比较,抗性品系的幼虫化蛹率明显提高,但羽化率无显著性差异.SDS-PAGE分析表明两品系的Ⅳ龄幼虫与成蚊蛋白条带均有明显区别:成蚊有分子量约为200 kDa的特异性条带,而幼虫则无;但两品系的幼虫、成蚊间的蛋白条带均无明显差异.
  • 摘要：本文利用光学显微镜和扫描电子显微镜,对内蒙古根河地区寄生于驯鹿皮下的鹿皮蝇Hypoderma diana Ⅱ、Ⅲ期幼虫进行了详细的形态学观察及描述,特别是在蝇蛆的伪头、体节刺和气门板结构上给出了重要特征.结果表明:Ⅱ期幼虫典型的形态特征是第1体节背面有一簇长棘,腹面有一簇短棘.而Ⅲ期幼虫伪头口裂背侧有两个小的退化肉质突起;第1体节背侧明显具横棘;第10体节腹面仅在后缘出现小棘,前缘裸;后气门呈对称"C"形,表面较平,气门环不闭合.扫描电镜观察发现光镜下所看到的所谓后气门板上圆形或椭圆形的"小气孔"并不是真正的气孔,而是一个较薄的透明薄膜状结构,膜中央有一和孔长轴平行的气缝.%The larval morphology of second and third instar Hypoderma diana, collected from reindeers of Genhe area of Inner Mongolia, especially the characters of the pseudocephalon, the somite spines and the structure of spiracular plates,were observed by optical microscope and scanning electron microscope ( SEM) . The results showed that the first segment of second instar larva had a long spines cluster on the dorsal part and a short spines cluster on the ventral part. But the third instar larva had marked transverse spines on dorsum of the first segment and small spines on the posterior margin of the tenth segment, while the anterior margin was free of spines. Besides, there were also two small fleshy, degenerated protuberances above oral fissure of pseudocephalon of the third instar larva. Their posterior spiracle was "C" in shape and symmetrical,with flat surface and incomplete annulus. The round or oval " small blowhole" on the spiracle, observed with optical microscope, was transparent memhrane with a crack in the centre confirmed by SEM. The present study provided an important reference for related teaching and scientific research.
  • 摘要：The mosquito populations were investigated by using C02 trap and larva collection in 5 villages and virgin forests around the villages in border between China and Laos. The results showed that a total of 8 762 individual mosquitoes belonging to 8 genus and 33 species were collected from the populated area, the 8 genus were Aedes. Culex, Anopheles,Mansonia , Coquillettidia , Armigeres , Uanotaenia and Mimomyia , among these mosquitoes , Cx. tritaeniorhynchus , An.sinensis and An. minimus were the dominant species; A total of 535 individual mosquitoes belonging to 9 genus and 46 species were collected from the virgin forests around the villages, the 9 genus were Aedes , Culex , Anopheles , Toxorhynchites ,Heizmannia, Topomyia, Malaya, Tripteroides and Uanotaenia , among these mosquitoes, Ae. albopictus was the dominant species. The diversity indices of mosquito communities were high and population structure was stable at both populated areas and forests.%为调查和了解云南省西双版纳中老边境蚊虫多样性状况,应用灯诱法和采集幼虫法对西双版纳州中老边境上5个村落和周边方圆4 km原始森林内的蚊虫的优势种组成、蚊虫群落结构的集中性指数、多样性指数和均匀度指数进行分析.结果显示,在居民区共采获蚊虫8 762只,共8属33种,隶属于蚊科Culicidae中的伊蚊属Aedes、库蚊属Culex、按蚊属Anopheles、曼蚊属Mansonia、柯蚊属Coquillettidia、阿蚊属Armigeres、蓝带蚊属Uanotaenia和小蚊属Mimomyia,其中优势种蚊虫为三带喙库蚊、中华按蚊和微小按蚊.在非居民区共采获蚊虫535只,共9属46种,隶属于蚊科Culicidae中的伊蚊属Aedes、库蚊属Culex、按蚊属Anopheles、巨蚊属Toxorhynchites、领蚊属Heizmannia、局限蚊属Topomyia、钩蚊属Malaya、杵蚊属Tripteroides和蓝带蚊属Uanotaenia,其中优势种蚊虫为白纹伊蚊.结果表明,居民区和非居民区其生物群落内种群结构稳定,均匀度较好.
  • 摘要：Many kinds of medical vectors and hosts settled in Fujian Province, where many natural foci of vector-horne diseases have been discovered. In pace with the economic construction being in progress on the Western Coast of the Taiwan Straits, the problems of medical vectors and vector-borne diseases should be taken seriously. The work of surveillance and quarantine of medical vectors should he strengthened in order to ensure the continued advance of the economic construction in the Western Coast of the Taiwan Straits.%福建的媒介生物和宿主动物种类丰富,存在许多虫媒病的自然疫源地或流行区.在进行海峡西岸经济区建设的同时,应密切关注虫媒传染病及其传播媒介,加强监测和检疫,以保障海峡西岸经济区的可持续发展.
  • 摘要：从甘肃景泰羊源脑多头蚴原头节提取总RNA,以Oligo(dT)纤维素柱纯化mRNA,利用Lambda ZAP II XR文库构建试剂盒构建了脑多头蚴cDNA表达文库.从构建的原始文库随机挑选单个噬菌斑进行PCR,确定文库重组率和插入外源基因片段大小,鉴定文库质量.结果表明脑多头蚴cDNA表达文库的原始库容量为1.0×106 pfu,扩增后文库滴度为1.6×109 pfu/mL.随机挑选的165个噬菌斑克隆中,0.25 kb以上的克隆155个,重组率为93.9%,对其中0.5 kb以上的115个克隆测序共获得104个表达序列标签(EST),分析后得到96个单一EST序列(Unique EST),其中20个EST与绦虫基因有同源性,38个EST与吸虫基因有同源性,4个EST与线虫基因有同源性,17个EST与其他物种有同源性,其余17个EST没有同源基因.这些EST编码的氨基酸序列有多头带绦虫六钩蚴Tm16抗原、猪带绦虫副肌球蛋白、猪带绦虫免疫原蛋白Ts76等绦虫抗原的同源蛋白以及烯醇化酶等一些酶类、热休克蛋白、肌动蛋白、核糖体蛋白等同源蛋白.%Total RNA was extracted from protoscolex of Coenurus cerebralis from naturally infected sheep of Jingtai Co. ,Gansu, and mRNA was isolated with Oligo ( dT) cellulose column. The Coenurus cerebralis cDNA expression library was constructed with Lambda ZAP Ⅱ XR vector. The randomly picked clones from primary cDNA library was amplified with PCR to detect the recombinant rate of cDNA lihrary and size of DNA inserts. The results indicated the size of the preliminary cDNA library was 1. 0 × 106 pfu with the titer of the amplified cDNA library of 1. 6 x 109 pfu/mL. From randomly picked 165 clones, 155 were with the size more than 250 bp and the recombinant rate was 93. 9％ . Furthermore , 115 clones sized more than 500 bp were sequenced and 104 expressed sequence tags (ESTs) were obtained. After analysis 96 unique ESTs were obtained, of which 20 were homologous with cestode, 38 were homologous with trematode, 4 were homologous with nematode, 17 ESTs showed similarity to known genes of other species, and 17 ESTs had no significant similarity to any sequences availahle in GenBank databases. Translation products of these ESTs were homologous with T. multiceps oncosphere protein Tm16, T. solium paramyosin, immunogen protein Ts76 and other Taenia antigens, enolase and other enzymes, heat shock protein, actin protein, rihosomal protein and so on.

    寄生虫与医学昆虫学报的期刊信息

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